H Chart1, G A Willshaw, T Cheasty. 1. Laboratory of Enteric Pathogens, Division of Gastrointestinal Infections, Central Public Health Laboratory, London, UK. hchart@phls.co.uk
Abstract
AIMS: To compare an experimental Reversed-Passive Latex Agglutination (RPLA) with Vero cells for the detection of Verocytotoxin expressed by VT-producing strains of Escherichia coli (VTEC). METHODS AND RESULTS: The RPLA was used alongside a Vero cell tissue culture assay for the detection of VT in bacterial culture supernatant fluids and patients' faecal extracts. CONCLUSION: The RPLA was comparable with the Vero cell assay, although slightly less sensitive. SIGNIFICANCE AND IMPACT OF THE STUDY: The RPLA test proved to be a simple, rapid and convenient method of detecting VT in bacterial culture supernatant fluids and in the faeces of patients infected with VTEC.
AIMS: To compare an experimental Reversed-Passive Latex Agglutination (RPLA) with Vero cells for the detection of Verocytotoxin expressed by VT-producing strains of Escherichia coli (VTEC). METHODS AND RESULTS: The RPLA was used alongside a Vero cell tissue culture assay for the detection of VT in bacterial culture supernatant fluids and patients' faecal extracts. CONCLUSION: The RPLA was comparable with the Vero cell assay, although slightly less sensitive. SIGNIFICANCE AND IMPACT OF THE STUDY: The RPLA test proved to be a simple, rapid and convenient method of detecting VT in bacterial culture supernatant fluids and in the faeces of patients infected with VTEC.
Authors: K C Carroll; K Adamson; K Korgenski; A Croft; R Hankemeier; J Daly; C H Park Journal: Eur J Clin Microbiol Infect Dis Date: 2003-10-23 Impact factor: 3.267