Literature DB >> 11399032

BIACORE data processing: an evaluation of the global fitting procedure.

M B Khalifa1, L Choulier, H Lortat-Jacob, D Altschuh, T Vernet.   

Abstract

Data from real-time molecular interaction analysis using BIACORE are currently evaluated by numerical integration. We have investigated the ability of two software packages (BIAevaluation 3.0 and CLAMP99) to analyze complex interactions. Three experimental data sets of high quality obtained with BIACORE upgraded and 2000 instruments, representative of simple bimolecular, heterogeneous ligand, and mass-transport-limited interactions, were processed by the global fitting procedure. The two software, which differ mainly in the statistical assessment of the output values, were able to discriminate correctly between various interacting models and provided very close output parameters with satisfactory statistical tests. Copyright 2001 Academic Press.

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Year:  2001        PMID： 11399032     DOI： 10.1006/abio.2001.5119

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  10 in total

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