Literature DB >> 11396305

Participation of thrombospondin-1 in the activation of latent transforming growth factor-beta in malignant glioma cells.

A Sasaki1, H Naganuma, E Satoh, T Kawataki, K Amagasaki, H Nukui.   

Abstract

Malignant glioma cells secrete transforming growth factor-beta (TGF-beta) and can activate latent TGF-beta. However, the mechanism of the latent TGF-beta activation has not yet been determined. This study examined whether thrombospondin-1 (TSP-1) secreted by malignant glioma cell lines participates in the activation of latent TGF-beta secreted by the glioma cells. Western blot analysis revealed that TSP-1 was present in both the cell lysates and the culture supernatants of all three malignant glioma cell lines (T98G, A172, and U251). A bioassay for TGF-beta activity revealed that all malignant glioma cell lines used in this study could activate latent TGF-beta by themselves. Latent TGF-beta 1 activation, evaluated by enzyme-linked immunosorbent assay, was inhibited by more than 50% by the addition of neutralizing anti-TSP-1 monoclonal antibody or anti-TSP-1 polyclonal antibody. These results indicate that TSP-1 has a predominant role in the activation of latent TGF-beta in malignant glioma cells.

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Year:  2001        PMID： 11396305     DOI： 10.2176/nmc.41.253

Source DB:  PubMed          Journal:  Neurol Med Chir (Tokyo)        ISSN： 0470-8105            Impact factor:   1.742


  7 in total

1.  Angiotensin II sustains brain inflammation in mice via TGF-beta.

Authors:  Tobias V Lanz; Zhaoqing Ding; Peggy P Ho; Jian Luo; Ankur N Agrawal; Hrishikesh Srinagesh; Robert Axtell; Hui Zhang; Michael Platten; Tony Wyss-Coray; Lawrence Steinman
Journal:  J Clin Invest       Date:  2010-07-12       Impact factor: 14.808

2.  Expression of syndecans, a heparan sulfate proteoglycan, in malignant gliomas: participation of nuclear factor-kappaB in upregulation of syndecan-1 expression.

Authors:  Arata Watanabe; Tadashi Mabuchi; Eiji Satoh; Koro Furuya; Lei Zhang; Shuichiro Maeda; Hirofumi Naganuma
Journal:  J Neurooncol       Date:  2006-03       Impact factor: 4.130

3.  Quantification of thrombospondin-1 secretion and expression of alphavbeta3 and alpha3beta1 integrins and syndecan-1 as cell-surface receptors for thrombospondin-1 in malignant glioma cells.

Authors:  Hirofumi Naganuma; Eiji Satoh; Takayuki Asahara; Kenichi Amagasaki; Arata Watanabe; Hiroki Satoh; Katsuhiro Kuroda; Lei Zhang; Hideaki Nukui
Journal:  J Neurooncol       Date:  2004-12       Impact factor: 4.130

Review 4.  Migratory potential of transplanted glial progenitors as critical factor for successful translation of glia replacement therapy: The gap between mice and men.

Authors:  Rohit K Srivastava; Jeff W M Bulte; Piotr Walczak; Miroslaw Janowski
Journal:  Glia       Date:  2017-12-20       Impact factor: 7.452

5.  Cell density regulates thrombospondin-1 production in malignant glioma cells.

Authors:  Hirofumi Naganuma; Eiji Satoh; Tomoyuki Kawataki; Kenichi Amagasaki; Hiroki Satoh; Hideaki Nukui
Journal:  J Neurooncol       Date:  2003-06       Impact factor: 4.130

6.  The neuroprotective functions of transforming growth factor beta proteins.

Authors:  Arpád Dobolyi; Csilla Vincze; Gabriella Pál; Gábor Lovas
Journal:  Int J Mol Sci       Date:  2012-07-03       Impact factor: 6.208

7.  Lactate-modulated induction of THBS-1 activates transforming growth factor (TGF)-beta2 and migration of glioma cells in vitro.

Authors:  Corinna Seliger; Petra Leukel; Sylvia Moeckel; Birgit Jachnik; Claudio Lottaz; Marina Kreutz; Alexander Brawanski; Martin Proescholdt; Ulrich Bogdahn; Anja-Katrin Bosserhoff; Arabel Vollmann-Zwerenz; Peter Hau
Journal:  PLoS One       Date:  2013-11-01       Impact factor: 3.240
  7 in total

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