| Literature DB >> 11394580 |
K Petrzik1, I Mráz, D Kubelková.
Abstract
The coat protein (CP) gene of Prunus necrotic ringspot virus (PNRSV) was cloned into pET 16b vector and expressed in Escherichia coli. CP-enriched fractions were prepared from whole cell lysate by differential centrifugation. The fraction sedimenting at 20,000 x g for 30 mins was used for preparation of a rabbit antiserum to CP. This antiserum had a titer of 1:2048 and reacted in a double-antibody sandwich ELISA (DAS-ELISA).Entities:
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Year: 2001 PMID： 11394580
Source DB: PubMed Journal: Acta Virol ISSN： 0001-723X Impact factor: 1.162