Three-dimensional echocardiography with tissue harmonic imaging shows excellent reproducibility in assessment of left ventricular volumes.

We studied the reproducibility of repeated measurements of left ventricular (LV) volumes by 2-dimensional (biplane method of disks) and 3-dimensional echocardiography (coaxial scanning) with tissue harmonic imaging. Ten healthy subjects underwent estimation of LV volumes by transthoracic echocardiography twice within 1 week by 2 different operators to investigate interexamination and operator variance. In addition, the analysis of LV volume was done manually by 2 observers to assess both interobserver and intraobserver variances. With 3D echocardiography, observer variation had the greatest impact on variance. Operator variability showed important contributions to total variance with the use of 2D echocardiography. The reproducibility of 3D echocardiography and tissue harmonic imaging is excellent and comparable to magnetic resonance imaging techniques; 3D echocardiography therefore should provide a powerful tool for noninvasive LV volume estimation.