Application of micellar electrokinetic capillary chromatography for quantitative analysis of quercetin in plant materials.

A simple method for the routine determination of quercetin in biological samples was developed. The method consists of hydrolysis of bonded quercetin, its isolation and preconcentration on solid-phase extraction (SPE) column and a final analytical step using micellar electrokinetic capillary chromatography. The working range, linear range, the limit of quantification, and the measurement uncertainty were tested in validation. The method is suitable for quercetin determination in fruit and vegetable samples.