BACKGROUND: Protein S-100beta is an established biochemical marker for cerebral injury in serum. For the further interpretation and possible use of S-100beta serum measurements in acute hepatic encephalopathy, renal elimination of S-100beta was measured in pigs with elevated S-100beta levels due to hepatic encephalopathy. METHODS: Eighteen female Norwegian Landrace pigs were randomly allocated to either hepatic devascularization (n=13) or sham operation (n=5). Repeated samples from the common carotid artery, right renal vein, and urine were simultaneously drawn for S-100beta analysis, using the Sangtec100 Liamat immunoassay. RESULTS: In hepatic devascularized pigs, arterial serum levels of S-100beta increased from 0.96+/-0.04 microg/L (mean +/- SEM) at t = 0h to 1.74+/-0.11 microg/L (mean +/- SEM) at t = 5 h. Urinary excretion increased simultaneously from 8.48+/-3.66 ng/h (mean +/- SEM) to 20.4+/-9.54 ng/h (mean +/- SEM), while renal arterial-venous fluxes for both kidneys increased from 1022+/-404 ng/h (mean +/- SEM) to 2444+/-590 ng/h (mean +/- SEM). CONCLUSIONS: Increased arterial S-100beta levels in pigs with acute hepatic encephalopathy are not a result of decreased renal elimination. The large difference between the renal arterial venous S-100beta concentrations and the urinary excretion of S-100beta indicate that renal metabolism is the major route of elimination.
BACKGROUND:Protein S-100beta is an established biochemical marker for cerebral injury in serum. For the further interpretation and possible use of S-100beta serum measurements in acute hepatic encephalopathy, renal elimination of S-100beta was measured in pigs with elevated S-100beta levels due to hepatic encephalopathy. METHODS: Eighteen female Norwegian Landrace pigs were randomly allocated to either hepatic devascularization (n=13) or sham operation (n=5). Repeated samples from the common carotid artery, right renal vein, and urine were simultaneously drawn for S-100beta analysis, using the Sangtec100 Liamat immunoassay. RESULTS: In hepatic devascularized pigs, arterial serum levels of S-100beta increased from 0.96+/-0.04 microg/L (mean +/- SEM) at t = 0h to 1.74+/-0.11 microg/L (mean +/- SEM) at t = 5 h. Urinary excretion increased simultaneously from 8.48+/-3.66 ng/h (mean +/- SEM) to 20.4+/-9.54 ng/h (mean +/- SEM), while renal arterial-venous fluxes for both kidneys increased from 1022+/-404 ng/h (mean +/- SEM) to 2444+/-590 ng/h (mean +/- SEM). CONCLUSIONS: Increased arterial S-100beta levels in pigs with acute hepatic encephalopathy are not a result of decreased renal elimination. The large difference between the renal arterial venous S-100beta concentrations and the urinary excretion of S-100beta indicate that renal metabolism is the major route of elimination.
Authors: Linda Papa; Linnet Akinyi; Ming Cheng Liu; Jose A Pineda; Joseph J Tepas; Monika W Oli; Wenrong Zheng; Gillian Robinson; Steven A Robicsek; Andrea Gabrielli; Shelley C Heaton; H Julia Hannay; Jason A Demery; Gretchen M Brophy; Joe Layon; Claudia S Robertson; Ronald L Hayes; Kevin K W Wang Journal: Crit Care Med Date: 2010-01 Impact factor: 7.598
Authors: Sambit Sen; Lars M Ytrebø; Christopher Rose; Ole-Martin Fuskevaag; Nathan A Davies; Geir I Nedredal; Roger Williams; Arthur Revhaug; Rajiv Jalan Journal: Intensive Care Med Date: 2004-01-21 Impact factor: 17.440
Authors: Linda Papa; Claudia S Robertson; Kevin K W Wang; Gretchen M Brophy; H Julia Hannay; Shelley Heaton; Ilona Schmalfuss; Andrea Gabrielli; Ronald L Hayes; Steven A Robicsek Journal: Neurocrit Care Date: 2015-02 Impact factor: 3.210
Authors: P Biberthaler; T Mussack; K-G Kanz; U Linsenmaier; K-J Pfeifer; W Mutschler; M Jochum Journal: Unfallchirurg Date: 2004-03 Impact factor: 1.000