Literature DB >> 11377719

Development and evaluation of a real-time quantitative PCR for the detection of human cytomegalovirus.

A M Kearns1, M Guiver, V James, J King.   

Abstract

A novel real-time quantitative PCR (QPCR) assay is described for monitoring CMV DNA load in clinical specimens using the LightCycler. The assay is rapid (< 40 min), easy to carry out, robust, reliable and is capable of detecting from 10 to over 2 x 10(5) CMV DNA copies with a wide linear range. Amplification and detection occur simultaneously, avoiding the need for post-PCR analysis and thereby minimising the risk of carryover contamination. The assay proved to be accurate, specific and reproducible when evaluated in three different laboratories. In addition, LightCycler results were comparable with those of TaqMan, an independent real-time QPCR assay.

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Year:  2001        PMID： 11377719     DOI： 10.1016/s0166-0934(01)00307-x

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  13 in total

1.  LightCycler-based quantitative PCR for detection of cytomegalovirus in blood, urine, and respiratory samples.

Authors:  A M Kearns; B Draper; W Wipat; A J Turner; J Wheeler; R Freeman; J Harwood; F K Gould; J H Dark
Journal:  J Clin Microbiol       Date:  2001-06       Impact factor: 5.948

Review 2.  Real-time PCR in clinical microbiology: applications for routine laboratory testing.

Authors:  M J Espy; J R Uhl; L M Sloan; S P Buckwalter; M F Jones; E A Vetter; J D C Yao; N L Wengenack; J E Rosenblatt; F R Cockerill; T F Smith
Journal:  Clin Microbiol Rev       Date:  2006-01       Impact factor: 26.132

3.  Real-time PCR diagnostics failure caused by nucleotide variability within exon 4 of the human cytomegalovirus major immediate-early gene.

Authors:  Martina Lengerova; Zdenek Racil; Pavlina Volfova; Jana Lochmanova; Jitka Berkovcova; Dana Dvorakova; Jiri Vorlicek; Jiri Mayer
Journal:  J Clin Microbiol       Date:  2007-01-17       Impact factor: 5.948

4.  Sequence diversity in the glycoprotein B gene complicates real-time PCR assays for detection and quantification of cytomegalovirus.

Authors:  Melinda B Nye; Adam R Leman; Michelle E Meyer; Marilyn A Menegus; Paul G Rothberg
Journal:  J Clin Microbiol       Date:  2005-10       Impact factor: 5.948

5.  Real-time PCR for detection and quantitation of leishmania in mouse tissues.

Authors:  Luc Nicolas; Eric Prina; Thierry Lang; Geneviève Milon
Journal:  J Clin Microbiol       Date:  2002-05       Impact factor: 5.948

6.  Development of a LightCycler PCR assay for detection and quantification of Aspergillus fumigatus DNA in clinical samples from neutropenic patients.

Authors:  Birgit Spiess; Dieter Buchheidt; Corinna Baust; Heyko Skladny; Wolfgang Seifarth; Udo Zeilfelder; Christine Leib-Mösch; Handan Mörz; Rüdiger Hehlmann
Journal:  J Clin Microbiol       Date:  2003-05       Impact factor: 5.948

7.  Development of real-time PCR assays for the quantitative detection of Epstein-Barr virus and cytomegalovirus, comparison of TaqMan probes, and molecular beacons.

Authors:  Jiska Jebbink; Xin Bai; Beverly Barton Rogers; D Brian Dawson; Richard H Scheuermann; Rana Domiati-Saad
Journal:  J Mol Diagn       Date:  2003-02       Impact factor: 5.568

8.  Antiviral responses following L-leucyl-L-leucine methyl esther (LLME)-treated lymphocyte infusions: graft-versus-infection without graft-versus-host disease.

Authors:  Joanne Filicko-O'Hara; Dolores Grosso; Phyllis R Flomenberg; Thea M Friedman; Janet Brunner; William Drobyski; Andres Ferber; Irina Kakhniashvili; Carolyn Keever-Taylor; Bijoyesh Mookerjee; Julie-An Talano; John I Wagner; Robert Korngold; Neal Flomenberg
Journal:  Biol Blood Marrow Transplant       Date:  2009-09-08       Impact factor: 5.742

9.  Killer immunoglobulin-like receptor gene repertoire influences viral load of primary human cytomegalovirus infection in renal transplant patients.

Authors:  D C Jones; S Peacock; D Hughes; J A Traherne; R L Allen; M C N M Barnardo; P Friend; C J Taylor; S Fuggle; J Trowsdale; N T Young
Journal:  Genes Immun       Date:  2014-09-25       Impact factor: 2.676

10.  Molecular detection of Theileria annulata infection in cattle by conventional PCR and quantitative real time PCR in India.

Authors:  Ahmed Magdy Selim; Manoranjan Das; Santosh Kumar Senapati; Geeta Rani Jena; Chinmoy Mishra; Indramani Nath; Shantibhusan Senapati; Manisha Sethi
Journal:  J Parasit Dis       Date:  2020-09-25
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