Literature DB >> 11376086

Detection of Aspergillus species DNA by PCR in bronchoalveolar lavage fluid.

M P Hayette1, D Vaira, F Susin, P Boland, G Christiaens, P Melin, P De Mol.   

Abstract

The usefulness of a nested PCR assay for detection of Aspergillus sp. DNA was evaluated in 177 bronchoalveolar lavage (BAL) fluid specimens. This test was accurate both to diagnose culture-negative BAL fluid specimens from patients with invasive pulmonary aspergillosis and to confirm culture-positive samples. However, it did not differentiate between infection and colonization.

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Year:  2001        PMID: 11376086      PMCID: PMC88140          DOI: 10.1128/JCM.39.6.2338-2340.2001

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  13 in total

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2.  A new sensitive sandwich enzyme-linked immunosorbent assay to detect galactofuran in patients with invasive aspergillosis.

Authors:  D Stynen; A Goris; J Sarfati; J P Latgé
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Review 3.  Aspergillus fumigatus and aspergillosis.

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Authors:  H Skladny; D Buchheidt; C Baust; F Krieg-Schneider; W Seifarth; C Leib-Mösch; R Hehlmann
Journal:  J Clin Microbiol       Date:  1999-12       Impact factor: 5.948

Review 5.  Aspergillosis.

Authors:  G P Bodey; S Vartivarian
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6.  Detection of Aspergillus species DNA in bronchoalveolar lavage samples by competitive PCR.

Authors:  S Bretagne; J M Costa; A Marmorat-Khuong; F Poron; C Cordonnier; M Vidaud; J Fleury-Feith
Journal:  J Clin Microbiol       Date:  1995-05       Impact factor: 5.948

7.  The detection of Aspergillus spp. by the polymerase chain reaction and its evaluation in bronchoalveolar lavage fluid.

Authors:  C M Tang; D W Holden; A Aufauvre-Brown; J Cohen
Journal:  Am Rev Respir Dis       Date:  1993-11

8.  Detection of Aspergillus fumigatus by polymerase chain reaction.

Authors:  C Spreadbury; D Holden; A Aufauvre-Brown; B Bainbridge; J Cohen
Journal:  J Clin Microbiol       Date:  1993-03       Impact factor: 5.948

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Authors:  W J Melchers; P E Verweij; P van den Hurk; A van Belkum; B E De Pauw; J A Hoogkamp-Korstanje; J F Meis
Journal:  J Clin Microbiol       Date:  1994-07       Impact factor: 5.948

10.  An Aspergillus fumigatus alkaline protease mutant constructed by gene disruption is deficient in extracellular elastase activity.

Authors:  C M Tang; J Cohen; D W Holden
Journal:  Mol Microbiol       Date:  1992-06       Impact factor: 3.501

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  17 in total

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Review 3.  Diagnostic accuracy of PCR alone compared to galactomannan in bronchoalveolar lavage fluid for diagnosis of invasive pulmonary aspergillosis: a systematic review.

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Review 4.  Molecular Diagnostic Testing for Aspergillus.

Authors:  Margaret V Powers-Fletcher; Kimberly E Hanson
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5.  Targeting novel genes for simultaneous detection of five fungal and bacterial agents from BAL samples using multiplex PCR assay.

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6.  Aspergillus galactomannan enzyme immunoassay and quantitative PCR for diagnosis of invasive aspergillosis with bronchoalveolar lavage fluid.

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Journal:  J Clin Microbiol       Date:  2004-12       Impact factor: 5.948

7.  Use of mycological, nested PCR, and real-time PCR methods on BAL fluids for detection of Aspergillus fumigatus and A. flavus in solid organ transplant recipients.

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8.  Development of a LightCycler PCR assay for detection and quantification of Aspergillus fumigatus DNA in clinical samples from neutropenic patients.

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9.  Rapid differentiation of Aspergillus species from other medically important opportunistic molds and yeasts by PCR-enzyme immunoassay.

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10.  Development and validation of a quantitative real-time PCR assay using fluorescence resonance energy transfer technology for detection of Aspergillus fumigatus in experimental invasive pulmonary aspergillosis.

Authors:  Cathal E O'Sullivan; Miki Kasai; Andrea Francesconi; Vidmantas Petraitis; Ruta Petraitiene; Amy M Kelaher; Alia A Sarafandi; Thomas J Walsh
Journal:  J Clin Microbiol       Date:  2003-12       Impact factor: 5.948

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