| Literature DB >> 11375929 |
Y Suzuki1, H Taira, T Tsunoda, J Mizushima-Sugano, J Sese, H Hata, T Ota, T Isogai, T Tanaka, S Morishita, K Okubo, Y Sakaki, Y Nakamura, A Suyama, S Sugano.
Abstract
Determination of the mRNA start site is the first step in identifying the promoter region, which is of key importance for transcriptional regulation of gene expression. The 'oligo-capping' method enabled us to introduce a sequence tag to the first base of an mRNA by replacing the cap structure of the mRNA. Using cDNA libraries made from oligo-capped mRNAs, we could identify the transcriptional start site of an individual mRNA just by sequencing the 5'-end of the cDNA. The fine mapping of transcriptional start sites was performed for 5880 mRNAs in 276 human genes. Contrary to our expectations, the majority of the genes showed a diverse distribution of transcriptional start sites. They were distributed over 61.7 bp with a standard deviation of 19.5. Our finding may reflect the dynamic nature of transcriptional initiation events of human genes in vivo.Entities:
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Year: 2001 PMID： 11375929 PMCID： PMC1083880 DOI： 10.1093/embo-reports/kve085
Source DB: PubMed Journal: EMBO Rep ISSN： 1469-221X Impact factor: 8.807