Literature DB >> 11375206

Quantitative PCR method for diagnosis of citrus bacterial canker.

J Cubero1, J H Graham, T R Gottwald.   

Abstract

For diagnosis of citrus bacterial canker by PCR, an internal standard is employed to ensure the quality of the DNA extraction and that proper requisites exist for the amplification reaction. The ratio of PCR products from the internal standard and bacterial target is used to estimate the initial bacterial concentration in citrus tissues with lesions.

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Year:  2001        PMID： 11375206      PMCID： PMC92950          DOI： 10.1128/AEM.67.6.2849-2852.2001

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  8 in total

1.  A simple extraction procedure for efficient routine detection of pathogenic bacteria in plant material by polymerase chain reaction.

Authors:  P Llop; P Caruso; J Cubero; C Morente; M M López
Journal:  J Microbiol Methods       Date:  1999-07       Impact factor: 2.363

2.  The polymerase chain reaction and plant disease diagnosis.

Authors:  J M Henson; R French
Journal:  Annu Rev Phytopathol       Date:  1993       Impact factor: 13.078

3.  Enhancement of the AMPLICOR enterovirus PCR test with a coprecipitant.

Authors:  E W Taggart; C L Byington; D R Hillyard; J E Robison; K C Carroll
Journal:  J Clin Microbiol       Date:  1998-11       Impact factor: 5.948

4.  The construction and use of a PCR internal control.

Authors:  P Sachadyn; J Kur
Journal:  Mol Cell Probes       Date:  1998-10       Impact factor: 2.365

Review 5.  Inhibition and facilitation of nucleic acid amplification.

Authors:  I G Wilson
Journal:  Appl Environ Microbiol       Date:  1997-10       Impact factor: 4.792

Review 6.  Technical aspects of quantitative competitive PCR.

Authors:  K Zimmermann; J W Mannhalter
Journal:  Biotechniques       Date:  1996-08       Impact factor: 1.993

7.  A simple and efficient PCR method for the detection of Agrobacterium tumefaciens in plant tumours.

Authors:  J Cubero; M C Martínez; P Llop; M M López
Journal:  J Appl Microbiol       Date:  1999-04       Impact factor: 3.772

8.  Detection of Xanthomonas campestris pv. citri by the polymerase chain reaction method.

Authors:  J S Hartung; J F Daniel; O P Pruvost
Journal:  Appl Environ Microbiol       Date:  1993-04       Impact factor: 4.792
  8 in total
  7 in total

1.  Ecological and Evolutionary Insights into Xanthomonas citri Pathovar Diversity.

Authors:  Kanika Bansal; Samriti Midha; Sanjeet Kumar; Prabhu B Patil
Journal:  Appl Environ Microbiol       Date:  2017-04-17       Impact factor: 4.792

2.  Genetic relationship among worldwide strains of Xanthomonas causing canker in citrus species and design of new primers for their identification by PCR.

Authors:  J Cubero; J H Graham
Journal:  Appl Environ Microbiol       Date:  2002-03       Impact factor: 4.792

3.  Using Genomics to Design a Pathovar-Specific Loop-Mediated Isothermal Amplification (LAMP) Assay, for the Improved Detection of Xanthomonas citri pv. citri.

Authors:  John Webster; Monica A Kehoe; Elisse Nogarotto; Linda Falconer; Nerida Jane Donovan; Toni A Chapman
Journal:  Microorganisms       Date:  2022-06-02

4.  Detection and diversity assessment of Xylella fastidiosa in field-collected plant and insect samples by using 16S rRNA and gyrB sequences.

Authors:  Jorge L M Rodrigues; M E Silva-Stenico; J E Gomes; J R S Lopes; S M Tsai
Journal:  Appl Environ Microbiol       Date:  2003-07       Impact factor: 4.792

5.  Detection and identification of Xanthomonas pathotypes associated with citrus diseases using comparative genomics and multiplex PCR.

Authors:  Natasha P Fonseca; Érica B Felestrino; Washington L Caneschi; Angélica B Sanchez; Isabella F Cordeiro; Camila G C Lemes; Renata A B Assis; Flávia M S Carvalho; Jesus A Ferro; Alessandro M Varani; José Belasque; Joao C Setubal; Guilherme P Telles; Deiviston S Aguena; Nalvo F Almeida; Leandro M Moreira
Journal:  PeerJ       Date:  2019-10-01       Impact factor: 2.984

6.  Development of a SYBR Green-based RT-qPCR assay for the detection of Indian citrus ringspot virus.

Authors:  Amol D Kokane; Kapil Lawrence; Sunil B Kokane; Mrugendra G Gubyad; Pragati Misra; M Krishna Reddy; Dilip Kumar Ghosh
Journal:  3 Biotech       Date:  2021-06-30       Impact factor: 2.893

7.  Comparison of Droplet Digital PCR and Quantitative PCR Assays for Quantitative Detection of Xanthomonas citri Subsp. citri.

Authors:  Yun Zhao; Qingyan Xia; Youping Yin; Zhongkang Wang
Journal:  PLoS One       Date:  2016-07-18       Impact factor: 3.240
  7 in total

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