M Kawakami1, J He, T Sakamoto, Y Okada. 1. Department of Traumatology and Critical Care Medicine, National Defense Medical College, Tokorozawa, Saitama, Japan. masato-kawakami@fc.kuh.kumamoto-u.ac.jp
Abstract
OBJECTIVE: To investigate the effects of catecholamines on the production of interleukin (IL)-6 and IL-1alpha in unburned skin after a burn injury. DESIGN: Randomized, controlled study. SETTING: Animal laboratory. SUBJECTS: Anesthetized female C57BL/6 mice. INTERVENTIONS: A full-thickness burn injury on 20% of total body surface area was produced on the clipped dorsum, which was followed by resuscitation with lactated Ringer's solution (0.1 mL/g). Phentolamine mesylate (10 mg/kg) or propranolol hydrochloride (10 mg/kg) was administered intraperitoneally before the burn injury. MEASUREMENTS AND MAIN RESULTS: Epinephrine and norepinephrine increased the production of IL-6 but not of IL-1alpha in normal abdominal skin, and these increases were reversed by a beta-blocker (propranolol hydrochloride) but not an alpha-blocker (phentolamine mesylate). A 20% full-thickness burn injury of the skin increased the plasma concentration of epinephrine 30 mins after the injury in female C57BL/6 mice, but not norepinephrine or dopamine. The burn also increased IL-6 production in unburned abdominal skin. The administration of a beta-blocker before a 20% burn injury suppressed the increased IL-6 production in unburned abdominal skin as determined by both protein level and mRNA expression. Pretreatment with the alpha-blocker was less effective. CONCLUSIONS: We propose that the neuroendocrine system activated by a 20% burn injury participates in the increased IL-6 production in the unburned skin.
OBJECTIVE: To investigate the effects of catecholamines on the production of interleukin (IL)-6 and IL-1alpha in unburned skin after a burn injury. DESIGN: Randomized, controlled study. SETTING: Animal laboratory. SUBJECTS: Anesthetized female C57BL/6 mice. INTERVENTIONS: A full-thickness burn injury on 20% of total body surface area was produced on the clipped dorsum, which was followed by resuscitation with lactated Ringer's solution (0.1 mL/g). Phentolamine mesylate (10 mg/kg) or propranolol hydrochloride (10 mg/kg) was administered intraperitoneally before the burn injury. MEASUREMENTS AND MAIN RESULTS:Epinephrine and norepinephrine increased the production of IL-6 but not of IL-1alpha in normal abdominal skin, and these increases were reversed by a beta-blocker (propranolol hydrochloride) but not an alpha-blocker (phentolamine mesylate). A 20% full-thickness burn injury of the skin increased the plasma concentration of epinephrine 30 mins after the injury in female C57BL/6 mice, but not norepinephrine or dopamine. The burn also increased IL-6 production in unburned abdominal skin. The administration of a beta-blocker before a 20% burn injury suppressed the increased IL-6 production in unburned abdominal skin as determined by both protein level and mRNA expression. Pretreatment with the alpha-blocker was less effective. CONCLUSIONS: We propose that the neuroendocrine system activated by a 20% burn injury participates in the increased IL-6 production in the unburned skin.
Authors: Min-Ho Kim; Farzam Gorouhi; Sandra Ramirez; Jennifer L Granick; Barbara A Byrne; Athena M Soulika; Scott I Simon; R Rivkah Isseroff Journal: J Invest Dermatol Date: 2013-10-11 Impact factor: 8.551