G X Dong1, Y P Feng. 1. Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Abstract
AIM: To explore if the inhibitory effect of 3-n-butylphthalide(NBP) on apoptosis induced by transient focal cerebral ischemia in rats is relevant to cortical calcineurin and calpain activities. METHODS: The model of cerebral ischemia-reperfusion was used. The activities of the two enzymes were measured by using biochemical methods. RESULTS: DL-NBP and D-NBP 20 mg.kg-1 were found to significantly reduce ischemia ipsilateral cortical calcineurin and calpain activities. However, L-NBP 20 mg.kg-1 showed no obvious effect. CONCLUSION: The anti-apoptotic effect of NBP may be relevant to its inhibition of calcineurin and calpain activities in focal cerebral ischemia rats.
AIM: To explore if the inhibitory effect of 3-n-butylphthalide(NBP) on apoptosis induced by transient focal cerebral ischemia in rats is relevant to cortical calcineurin and calpain activities. METHODS: The model of cerebral ischemia-reperfusion was used. The activities of the two enzymes were measured by using biochemical methods. RESULTS:DL-NBP and D-NBP 20 mg.kg-1 were found to significantly reduce ischemia ipsilateral cortical calcineurin and calpain activities. However, L-NBP 20 mg.kg-1 showed no obvious effect. CONCLUSION: The anti-apoptotic effect of NBP may be relevant to its inhibition of calcineurin and calpain activities in focal cerebral ischemiarats.