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Literature DB >> 11370872

The wild-type conformation of the Mos-1 inverted terminal repeats is suboptimal for transposition in bacteria.

C Augé-Gouillou¹, M H Hamelin, M V Demattei, M Periquet, Y Bigot.

Abstract

The two inverted terminal repeats (ITRs) flanking the Mos-1 mariner element differ in sequence at four positions. Gel retardation experiments indicated that each of these differences has a significant impact on the quality of the interaction between the ITR and the Mos-1 transposase. We showed that the transposase binds to the 3' ITR better than to the 5' ITR. The results of transposition assays performed in Escherichia coli indicated that these differences have an influence on the rate of transposition and the stability of the transposition products. Finally, we find that the wild-type configuration of the Mos-1 element, with one 5' ITR and one 3' ITR, is less efficient for transposition in bacteria than that of an element having two 3' ITRs.

Entities: Disease Species

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Year: 2001 PMID： 11370872 DOI： 10.1007/s004380000385

Source DB: PubMed Journal: Mol Genet Genomics ISSN： 1617-4623 Impact factor: 3.291

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Cited

28 in total

1. DNA-binding activity and subunit interaction of the mariner transposase.

Authors: L Zhang; A Dawson; D J Finnegan
Journal: Nucleic Acids Res Date: 2001-09-01 Impact factor: 16.971

2. PIF- and Pong-like transposable elements: distribution, evolution and relationship with Tourist-like miniature inverted-repeat transposable elements.

Authors: Xiaoyu Zhang; Ning Jiang; Cédric Feschotte; Susan R Wessler
Journal: Genetics Date: 2004-02 Impact factor: 4.562

The wild-type conformation of the Mos-1 inverted terminal repeats is suboptimal for transposition in bacteria.

1. DNA-binding activity and subunit interaction of the mariner transposase.

2. PIF- and Pong-like transposable elements: distribution, evolution and relationship with Tourist-like miniature inverted-repeat transposable elements.

3. Birth of a chimeric primate gene by capture of the transposase gene from a mobile element.

4. Population genetics models of competition between transposable element subfamilies.

Review 5. Applying mobile genetic elements for genome analysis and evolution.

Review 6. Mariner transposons as genetic tools in vertebrate cells.

7. The bacterial Tn9 chloramphenicol resistance gene: an attractive DNA segment for Mos1 mariner insertions.

8. Internal deletions of transposable elements: the case of Lemi elements.

9. Bias between the left and right inverted repeats during IS911 targeted insertion.

Review 10. Bacterial genetic methods to explore the biology of mariner transposons.