| Literature DB >> 11369872 |
Robert E Lanford1, Helen Lee1, Deborah Chavez1, Bernadette Guerra1, Kathleen M Brasky1.
Abstract
A full-length cDNA clone of the hepatitis C virus (HCV) genotype 1 prototype (subtype 1a) sequence was constructed. Synthetic RNA produced from the initial cDNA clone was not infectious following intrahepatic inoculation of a chimpanzee. A consensus clone was prepared by comparison with multiple full-length HCV sequences of genotypes 1, 2 and 3. A total of 11 non-consensus amino acid residues were altered by mutagenesis. Synthetic RNA from the repaired clone initiated a typical, acute-resolving HCV infection following intrahepatic inoculation of a chimpanzee. In addition, at least one of three chimeric cDNA clones constructed between the HCV-1 and H77 genotype 1a strains of HCV was infectious in a chimpanzee. This is the first example of an infectious chimeric HCV clone. An infectious cDNA clone of HCV-1 will be of particular value, since it is the prototype HCV sequence and many commonly used reagents are based on this sequence.Entities:
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Year: 2001 PMID： 11369872 DOI： 10.1099/0022-1317-82-6-1291
Source DB: PubMed Journal: J Gen Virol ISSN： 0022-1317 Impact factor: 3.891