| Literature DB >> 11358845 |
W Szeto1, W Jiang, D A Tice, B Rubinfeld, P G Hollingshead, S E Fong, D L Dugger, T Pham, D G Yansura, T A Wong, J C Grimaldi, R T Corpuz, J S Singh, G D Frantz, B Devaux, C W Crowley, R H Schwall, D A Eberhard, L Rastelli, P Polakis, D Pennica.
Abstract
Genetic defects in the Wnt-1 signaling pathway contribute to human tumor progression and are especially prevalent in colorectal cancer. We screened mouse C57MG cells to isolate mRNAs induced by Wnt-1 and identified Stra6, an mRNA known to be up-regulated by retinoic acid. Up-regulation of Stra6 mRNA was also observed in hyperplastic mammary tissue and mammary gland tumors from transgenic mice expressing Wnt-1 and in human tumors that frequently harbor defects in Wnt-1 signaling. Stimulation of C57MG cells with retinoic acid plus Wnt-1 resulted in expression of Stra6 transcript to levels greatly exceeding that observed with either stimulus alone. This synergy could be explained in part by the up-regulation of retinoic acid receptor-gamma that was observed in response to Wnt-1 signaling. Accordingly, treatment of human colorectal cancer cell lines with retinoic acid resulted in the up-regulation of Stra6 mRNA and accumulation of Stra6 protein at the cell membrane. The data support a model in which Wnt-1 signaling synergizes with retinoids to activate retinoic acid receptor-gamma-responsive genes in human cancers.Entities:
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Year: 2001 PMID： 11358845
Source DB: PubMed Journal: Cancer Res ISSN： 0008-5472 Impact factor: 12.701