| Literature DB >> 11354477 |
H Kojima1, M Hirotani, N Nakatsubo, K Kikuchi, Y Urano, T Higuchi, Y Hirata, T Nagano.
Abstract
Diaminofluoresceins are widely used for detection and imaging of nitric oxide (NO), but for biological applications, they have the disadvantages that the fluorescence of the fluorescein chromophore is pH-sensitive and overlaps the autofluorescence of cells. We have developed a membrane-permeable fluorescent indicator for NO based on the rhodamine chromophore, DAR-4M AM, which can be excited with 550-nm light. The fluorescence quantum yield of the product after reaction with NO is 840 times higher than that of DAR-4M. The detection limit of NO was 7 nM, and the fluorescence showed no pH dependency above pH 4. DAR-4M AM was successfully applied to practical bioimaging of NO produced in bovine aortic endothelial cells.Entities:
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Year: 2001 PMID： 11354477 DOI： 10.1021/ac001136i
Source DB: PubMed Journal: Anal Chem ISSN： 0003-2700 Impact factor: 6.986