Literature DB >> 11353649

Staphylococcus aureus cell extract transcription-translation assay: firefly luciferase reporter system for evaluating protein translation inhibitors.

R W Murray1, E P Melchior, J C Hagadorn, K R Marotti.   

Abstract

The promoter for the Staphylococcus aureus capsule polysaccharide synthesis gene (cap1A) was cloned in front of the firefly luciferase gene for use in a cell extract S. aureus transcription-translation system. The assay is rapid, reproducible, and sensitive and has a lower background level than the radiolabeled amino acid incorporation translation assays. We present data evaluating a transcription inhibitor and a number of protein translation inhibitors in this system.

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Year:  2001        PMID： 11353649      PMCID： PMC90569          DOI： 10.1128/AAC.45.6.1900-1904.2001

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  4 in total

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Authors:  R P Novick
Journal:  Methods Enzymol       Date:  1991       Impact factor: 1.600

2.  An in vitro coupled transcription-translation system from Staphylococcus aureus.

Authors:  R Mahmood; P Compagnone-Post; S A Khan
Journal:  Gene       Date:  1991-09-30       Impact factor: 3.688

Review 3.  In vitro synthesis of protein in microbial systems.

Authors:  G Zubay
Journal:  Annu Rev Genet       Date:  1973       Impact factor: 16.830

4.  Cloning of genes affecting capsule expression in Staphylococcus aureus strain M.

Authors:  C Y Lee
Journal:  Mol Microbiol       Date:  1992-06       Impact factor: 3.501
  4 in total
  20 in total

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2.  Discovery and characterization of a novel class of pyrazolopyrimidinedione tRNA synthesis inhibitors.

Authors:  Justin I Montgomery; James F Smith; Andrew P Tomaras; Richard Zaniewski; Craig J McPherson; Laura A McAllister; Sandra Hartman-Neumann; Joel T Arcari; Marykay Lescoe; Jemy Gutierrez; Ying Yuan; Chris Limberakis; Alita A Miller
Journal:  J Antibiot (Tokyo)       Date:  2014-12-03       Impact factor: 2.649

3.  RNA antitoxin SprF1 binds ribosomes to attenuate translation and promote persister cell formation in Staphylococcus aureus.

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4.  Discovery and Analysis of Natural-Product Compounds Inhibiting Protein Synthesis in Pseudomonas aeruginosa.

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Journal:  Antimicrob Agents Chemother       Date:  2016-07-22       Impact factor: 5.191

5.  Structural insights into species-specific features of the ribosome from the pathogen Staphylococcus aureus.

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Journal:  Proc Natl Acad Sci U S A       Date:  2015-10-13       Impact factor: 11.205

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7.  Microscale Adaptation of In Vitro Transcription/Translation for High-Throughput Screening of Natural Product Extract Libraries.

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Journal:  Antimicrob Agents Chemother       Date:  2008-04-21       Impact factor: 5.191

9.  Stimulators of translation identified during a small molecule screening campaign.

Authors:  Unkyung Shin; David E Williams; Dima Kozakov; David R Hall; Dmitri Beglov; Sandor Vajda; Raymond J Andersen; Jerry Pelletier
Journal:  Anal Biochem       Date:  2014-02-15       Impact factor: 3.365

10.  Ribosomal alterations contribute to bacterial resistance against the dipeptide antibiotic TAN 1057.

Authors:  E Limburg; R Gahlmann; H-P Kroll; D Beyer
Journal:  Antimicrob Agents Chemother       Date:  2004-02       Impact factor: 5.191
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