OBJECTIVES: In order to study the possible role of nitric oxide (NO) in the development of periodontitis, we measured the concentration of its stable metabolite nitrite (NO2-) in the saliva of patients with periodontitis and healthy subjects. MATERIALS AND METHODS: We have analysed salivary NO2- concentrations in 25 subjects with rapidly progressive periodontitis (RPP), 25 with adult periodontitis (AP) and in 25 periodontally-healthy persons. The concentrations of NO2- were determined by the Griess reaction in microtitration plates. Periodontal tissue destruction was determined by measuring the attachment level loss using standard methods. RESULTS: Subjects with periodontitis had significantly less NO2- in saliva than healthy subjects. Subjects with RPP had lower NO2- concentrations than those with AP Parotid gland saliva contained less NO2- than sublingual gland or total saliva. CONCLUSIONS: Local NO production is decreased in patients with periodontitis. This effect is more pronounced in those with severe types of disease.
OBJECTIVES: In order to study the possible role of nitric oxide (NO) in the development of periodontitis, we measured the concentration of its stable metabolite nitrite (NO2-) in the saliva of patients with periodontitis and healthy subjects. MATERIALS AND METHODS: We have analysed salivary NO2- concentrations in 25 subjects with rapidly progressive periodontitis (RPP), 25 with adult periodontitis (AP) and in 25 periodontally-healthy persons. The concentrations of NO2- were determined by the Griess reaction in microtitration plates. Periodontal tissue destruction was determined by measuring the attachment level loss using standard methods. RESULTS: Subjects with periodontitis had significantly less NO2- in saliva than healthy subjects. Subjects with RPP had lower NO2- concentrations than those with AP Parotid gland saliva contained less NO2- than sublingual gland or total saliva. CONCLUSIONS: Local NO production is decreased in patients with periodontitis. This effect is more pronounced in those with severe types of disease.
Authors: Bruno S Herrera; Rodrigo Martins-Porto; Paula Campi; Marinella Holzhausen; Simone A Teixeira; Gustavo D Mendes; Soraia K P Costa; Robert Gyurko; Thomas E Van Dyke; Luis C Spolidório; Marcelo N Muscará Journal: Arch Oral Biol Date: 2010-09-22 Impact factor: 2.633
Authors: Frank Schreiber; Peter Stief; Armin Gieseke; Ines M Heisterkamp; Willy Verstraete; Dirk de Beer; Paul Stoodley Journal: BMC Biol Date: 2010-03-22 Impact factor: 7.431
Authors: Hani S AlMoharib; Abdulrahman AlMubarak; Raed AlRowis; Amrita Geevarghese; R S Preethanath; Sukumaran Anil Journal: J Int Oral Health Date: 2014-07