L-glutamic acid production in a novel three phase fluidized bed reactor using coimmobilized bio-catalyst.

The production of L-Glutamic acid has been studied using coimmobilized whole cells of pseudomonas reptilivora and micrococcus glutamicus in a three phase fluidized bed bioreactor with selected production medium enriched with glucose. The effect of initial substrate concentration, temperature, pH and aeration rate on the yield of glutamic acid has been investigated. It has been found that the acid production increases exponentially with substrate concentration and mass transfer co-efficient varied linearly with temperature and aeration rate. The optimum temperature and pH are 31 degree Celsius and 7.2 respectively.