Literature DB >> 113426

Rickettsial hemolysis: rapid method for enumeration of metabolically active typhus rickettsiae.

T S Walker, H H Winkler.   

Abstract

A new assay is described for enumerating biologically active typhus rickettsiae (Madrid E strain), based on adsorption of rickettsiae to erythrocytes in the presence of NaF (which allows adsorption but not lysis) and lysis in the presence of anti-Rickettsia prowazeki immune serum (which allows only a single round of lysis). The number of lysed erythrocytes is then used to estimate the number of active rickettsiae.

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Year:  1979        PMID： 113426      PMCID： PMC275367          DOI： 10.1128/jcm.9.5.645-647.1979

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  8 in total

1.  Metabolic studies of rickettsiae. I. The effects of antimicrobial substances and enzyme inhibitors on the oxidation of glutamate by purified rickettsiae.

Authors:  C L WISSEMAN; E B JACKSON; F E HAHN; A C LEY; J E SMADEL
Journal:  J Immunol       Date:  1951-08       Impact factor: 5.422

2.  Method for counting Rickettsiae and Chlamydiae in purified suspensions.

Authors:  R Silberman; P Fiset
Journal:  J Bacteriol       Date:  1968-01       Impact factor: 3.490

3.  Rickettsial hemolysis: adsorption, desorption, readsorption, and hemagglutination.

Authors:  H H Winkler
Journal:  Infect Immun       Date:  1977-09       Impact factor: 3.441

4.  Limits of rickettsial infectivity.

Authors:  R Ormsbee; M Peacock; R Gerloff; G Tallent; D Wike
Journal:  Infect Immun       Date:  1978-01       Impact factor: 3.441

5.  Adsorption of typhus rickettsiae to ghosts of sheep erythrocytes.

Authors:  H H Winkler; L E Ramm
Journal:  Infect Immun       Date:  1975-06       Impact factor: 3.441

6.  Studies of the rickettsial plaque assay technique.

Authors:  D A Wike; G Tallent; M G Peacock; R A Ormsbee
Journal:  Infect Immun       Date:  1972-05       Impact factor: 3.441

7.  Rickettsial hemolysis: effect of metabolic inhibitors upon hemolysis and adsorption.

Authors:  L E Ramm; H H Winkler
Journal:  Infect Immun       Date:  1973-04       Impact factor: 3.441

8.  In vitro studies on Rickettsia-host cell interactions: lag phase in intracellular growth cycle as a function of stage of growth of infecting Rickettsia prowazeki, with preliminary observations on inhibition of rickettsial uptake by host cell fragments.

Authors:  C L Wisseman; A D Waddell; D J Silverman
Journal:  Infect Immun       Date:  1976-06       Impact factor: 3.441
  8 in total
  33 in total

1.  Rickettsia prowazekii methionine aminopeptidase as a promising target for the development of antibacterial agents.

Authors:  Travis R Helgren; Congling Chen; Phumvadee Wangtrakuldee; Thomas E Edwards; Bart L Staker; Jan Abendroth; Banumathi Sankaran; Nicole A Housley; Peter J Myler; Jonathon P Audia; James R Horn; Timothy J Hagen
Journal:  Bioorg Med Chem       Date:  2016-11-10       Impact factor: 3.641

2.  Acquisition of glucose by Rickettsia prowazekii through the nucleotide intermediate uridine 5'-diphosphoglucose.

Authors:  H H Winkler; R M Daugherty
Journal:  J Bacteriol       Date:  1986-09       Impact factor: 3.490

3.  Rickettsia prowazekii requires host cell serine and glycine for growth.

Authors:  F E Austin; J Turco; H H Winkler
Journal:  Infect Immun       Date:  1987-01       Impact factor: 3.441

4.  Rickettsial interactions with human endothelial cells in vitro: adherence and entry.

Authors:  T S Walker
Journal:  Infect Immun       Date:  1984-05       Impact factor: 3.441

5.  Rickettsial effects on leukotriene and prostaglandin secretion by mouse polymorphonuclear leukocytes.

Authors:  T S Walker; C S Hoover
Journal:  Infect Immun       Date:  1991-01       Impact factor: 3.441

6.  Synthesis of DNA, rRNA, and protein by Rickettsia prowazekii growing in untreated or gamma interferon-treated mouse L929 cells.

Authors:  Q Gao; J Turco; H H Winkler
Journal:  Infect Immun       Date:  1993-06       Impact factor: 3.441

7.  Effect of mouse lymphokines and cloned mouse interferon-gamma on the interaction of Rickettsia prowazekii with mouse macrophage-like RAW264.7 cells.

Authors:  J Turco; H H Winkler
Journal:  Infect Immun       Date:  1984-08       Impact factor: 3.441

8.  Activated complex of L-cells and Rickettsia prowazekii with N-ethylmaleimide-insensitive phospholipase A.

Authors:  H H Winkler; E T Miller
Journal:  Infect Immun       Date:  1984-09       Impact factor: 3.441

9.  Role of the nitric oxide synthase pathway in inhibition of growth of interferon-sensitive and interferon-resistant Rickettsia prowazekii strains in L929 cells treated with tumor necrosis factor alpha and gamma interferon.

Authors:  J Turco; H H Winkler
Journal:  Infect Immun       Date:  1993-10       Impact factor: 3.441

10.  Effect of gamma interferon on phospholipid hydrolysis and fatty acid incorporation in L929 cells infected with Rickettsia prowazekii.

Authors:  H H Winkler; L Day; R Daugherty; J Turco
Journal:  Infect Immun       Date:  1993-08       Impact factor: 3.441
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