| Literature DB >> 11342111 |
K Nosaka1, M Onozuka, N Kakazu, S Hibi, H Nishimura, H Nishino, T Abe.
Abstract
A human thiamine pyrophosphokinase cDNA clone (hTPK1) was isolated and sequenced. When the intact hTPK1 open reading frame was expressed as a histidine-tag fusion protein in Escherichia coli, marked enzyme activity was detected in the bacterial cells. The hTPK1 mRNA was widely expressed in various human tissues at a very low level, and the mRNA content in cultured fibroblasts was unaffected by the thiamine concentration of the medium. The chromosome localization of the hTPK1 gene was assigned to 7q34.Entities:
Mesh：
Substances：
Year: 2001 PMID： 11342111 DOI： 10.1016/s0167-4781(00)00247-5
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002