| Literature DB >> 11339280 |
R Bongiovanni1, B K Yamamoto, G E Jaskiw.
Abstract
A high-performance liquid chromatographic method for measuring neutral amino acids in rat sera, brain tissues, and perfusates was developed by using o-phthalaldehyde sulfite as a pre-column derivatization reagent. With the present method, it was possible to separate the neutral amino acids within a single run in 25 min, while the acidic amino acids were eluted near or at the solvent front. The recovery was above 88.8% with a relative standard deviation (RSD) below 4.2%. The within- and between-day assay reproducibility for the determination of rat serum amino acids showed RSDs below 1.35 and 7.61%, respectively. In the present study, the neutral amino acids were assayed with high sensitivity, accuracy and good reproducibility in a relatively short time and on a small sample size.Entities:
Mesh：
Substances：
Year: 2001 PMID： 11339280 DOI： 10.1016/s0378-4347(00)00629-0
Source DB: PubMed Journal: J Chromatogr B Biomed Sci Appl ISSN： 1387-2273