| Literature DB >> 11334739 |
R Gauderon1, P B Lukins, C J Sheppard.
Abstract
We describe the principles and characteristics of second-harmonic generation imaging (SHGI) and explore various methods for optimization of the technique. Second-harmonic imaging is optimized for ultrashort laser pulses, high numerical aperture microscope objectives, a highly sensitive non-descanned large area detector, pseudo-phase-matching, and specimens with large second-order non linearity or which exhibit surface plasmon enhanced phenomena. We also compare and contrast the techniques of SHGI and two-photon excited fluorescence imaging.Year: 2001 PMID: 11334739 DOI: 10.1016/s0968-4328(00)00066-4
Source DB: PubMed Journal: Micron ISSN: 0968-4328 Impact factor: 2.251