| Literature DB >> 11334369 |
S Saito1, H Yamauchi, Y Hasui, J Kurashige, H Ochi, K Yoshida.
Abstract
In order to establish a quantitative assay for 8-OHdG concentrations in urine, we examined the precision of a test for the recovery of 8-OHdG in urine by using an ELISA method. The coefficient of variation (CV) for assay with incubation in water or air ranged between 7.0% and 8.4% and between 19.2% and 30.6%, respectively. The data by using incubation in water gave higher accuracy than those in air. The recovery rates of 8-OHdG in urine sample ranged from 95 - 114%. Our results indicated that the excellent sensitivity of this ELISA kit by using incubation in water makes its use possible for the determination in urine with a good reproducibility and recovery of 8-OHdG-spiked samples.Entities:
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Year: 2000 PMID： 11334369
Source DB: PubMed Journal: Res Commun Mol Pathol Pharmacol ISSN： 1078-0297