Possible involvement of mGluR1 together with group II and III mGluRs in ibotenate-stimulated cAMP formation in the rat brain cortical slices.

The mechanism of adenylyl cyclase activation by the stimulation of metabotropic glutamate receptors (mGluRs) is still unknown. Our previous studies have shown that mGluR agonist, ibotenic acid, produced a significant increase in cAMP accumulation. The aim of the present studies was to investigate the mechanism of ibotenate-stimulated cAMP formation in cortical slices of adult rats. Antagonists of all groups of mGluRs were examined to establish their effects on the stimulation of cAMP production by ibotenic acid. The obtained results indicate that ibotenate-stimulated cAMP accumulation in the rat brain cortical slices depends on the activation of mGluR1, but not on mGluR5. Moreover, activation of group II and group III mGluRs also influences ibotenate-stimulated cAMP formation.