Luminol-dependent chemiluminescence analysis of chitooligosaccharide-induced rapid production of hydrogen peroxide by intact wheat seedlings.

The feasibility of a luminol-dependent chemiluminescence analysis of hydrogen peroxide production by intact wheat seedlings using a KhL-003 chemiluminometer was determined. It was shown that the minimal H2O2 concentration that can be detected in a 0.5-ml sample with this instrument is 0.125 microM. Analysis of biological activity of a mixture of chitooligosaccharides with molecular masses from 5 to 10 kD and acetylation degree of 65% demonstrated that, at a concentration of 1 microg/ml, they induce rapid overproduction of H2O2 in roots of 3-day-old wheat seedlings.