Literature DB >> 11332751

Quantifying DNA damage by gel electrophoresis, electronic imaging and number-average length analysis.

J C Sutherland1, D C Monteleone, J G Trunk, P V Bennett, B M Sutherland.   

Abstract

DNA damages that can be converted to single- or double strand breaks can be quantified by separating DNA by gel electrophoresis and obtaining a quantitative image of the resulting distribution of DNA in the gel. We review the theory of this method and discuss its implementation, including the charge-coupled device (CCD) camera systems we developed to acquire images of fluorophore labeled DNA.

Entities:  

Mesh：

Substances：

Year:  2001        PMID： 11332751     DOI： 10.1002/1522-2683()22:5<843::AID-ELPS843>3.0.CO;2-9

Source DB:  PubMed          Journal:  Electrophoresis        ISSN： 0173-0835            Impact factor:   3.535


  7 in total

1.  Quantifying double-strand breaks and clustered damages in DNA by single-molecule laser fluorescence sizing.

Authors:  Elena M Filippova; Denise C Monteleone; John G Trunk; Betsy M Sutherland; Stephen R Quake; John C Sutherland
Journal:  Biophys J       Date:  2003-02       Impact factor: 4.033

2.  Effective and robust plasmid topology analysis and the subsequent characterization of the plasmid isoforms thereby observed.

Authors:  Michael J Molloy; Valerie S Hall; Susannah I Bailey; Katharine J Griffin; Jim Faulkner; Mark Uden
Journal:  Nucleic Acids Res       Date:  2004-09-09       Impact factor: 16.971

3.  Processing of bistranded abasic DNA clusters in gamma-irradiated human hematopoietic cells.

Authors:  Alexandros G Georgakilas; Paula V Bennett; David M Wilson; Betsy M Sutherland
Journal:  Nucleic Acids Res       Date:  2004-10-19       Impact factor: 16.971

4.  Endogenous DNA damage clusters in human skin, 3-D model, and cultured skin cells.

Authors:  Paula V Bennett; Noelle L Cuomo; Sunirmal Paul; Stefan T Tafrov; Betsy M Sutherland
Journal:  Free Radic Biol Med       Date:  2005-09-15       Impact factor: 7.376

5.  DOE program--developing a scientific basis for responses to low-dose exposures: impact on dose-response relationships.

Authors:  Antone L Brooks; Lezlie Couch
Journal:  Dose Response       Date:  2006-09-23       Impact factor: 2.658

6.  High efficiency detection of bi-stranded abasic clusters in gamma-irradiated DNA by putrescine.

Authors:  Alexandros G Georgakilas; Paula V Bennett; Betsy M Sutherland
Journal:  Nucleic Acids Res       Date:  2002-07-01       Impact factor: 16.971

7.  Human abasic endonuclease action on multilesion abasic clusters: implications for radiation-induced biological damage.

Authors:  Brigitte Paap; David M Wilson; Betsy M Sutherland
Journal:  Nucleic Acids Res       Date:  2008-03-19       Impact factor: 16.971
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.