A method for the separation of plasma amino acids by ion-exchange chromatography combined with a semi-automatic computation of plasma amino acid concentrations.

A method is described for combining the separation of plasma amino acids on a single column amino acid analyzer with semi-automatic computation of the plasma amino acid concentrations. The system used for the quantitation of the amino acids consists of an integrator with a teletype printer fitted with a puncher, and a table calculator with a punch reader and a converter. By using this system, the total time required for the calculation of the molar plasma concentration of 30 amino acids from one analytical run is less than 10 min.