X W Zhang1, J F Jiang, B Xu. 1. Department of Pharmacology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 200031, China.
Abstract
AIM: To study the mechanism of differentiation-inducing action of 10-hydroxycamptothecin (HCPT) on human hepatoma Hep G2 cells. METHODS: The proliferating cell nuclear antigen (PCNA) expression was studied by immunocytochemical staining method. The cell cycle distribution and the wild-type protein p53 expression were measured by flow cytometry. Telomerase activity was assayed with telomeric repeat amplification protocol (TRAP). RESULTS: After treatment with HCPT at differentiation-inducing concentrations 5-20 micrograms.L-1 for 6 d, Hep G2 cells were mainly arrested at G2/M phase and the PCNA expression rate was lower than that of control cells. When Hep G2 cells grew in a medium containing HCPT 5 micrograms.L-1 for 6 d, the p53 expression level markedly increased in comparison with the control cells. The telomerase activity did not change in Hep G2 cells treated with HCPT 5-20 micrograms.L-1 for 8 d. CONCLUSION: The differentiation-inducing effect of HCPT on Hep G2 cells is related with the cell cycle arrest at G2/M phase, down-regulation of PCNA and up-regulation of wild-type protein p53.
AIM: To study the mechanism of differentiation-inducing action of 10-hydroxycamptothecin (HCPT) on humanhepatoma Hep G2 cells. METHODS: The proliferating cell nuclear antigen (PCNA) expression was studied by immunocytochemical staining method. The cell cycle distribution and the wild-type protein p53 expression were measured by flow cytometry. Telomerase activity was assayed with telomeric repeat amplification protocol (TRAP). RESULTS: After treatment with HCPT at differentiation-inducing concentrations 5-20 micrograms.L-1 for 6 d, Hep G2 cells were mainly arrested at G2/M phase and the PCNA expression rate was lower than that of control cells. When Hep G2 cells grew in a medium containing HCPT 5 micrograms.L-1 for 6 d, the p53 expression level markedly increased in comparison with the control cells. The telomerase activity did not change in Hep G2 cells treated with HCPT 5-20 micrograms.L-1 for 8 d. CONCLUSION: The differentiation-inducing effect of HCPT on Hep G2 cells is related with the cell cycle arrest at G2/M phase, down-regulation of PCNA and up-regulation of wild-type protein p53.
Authors: Xiong Min; Han Heng; Hua-Long Yu; Mao Dan; Chen Jie; Yun Zeng; He Ning; Zhi-Gang Liu; Zhi-Yong Wang; Wang Lin Journal: Oncol Lett Date: 2017-12-13 Impact factor: 2.967
Authors: Anthony A Attama; Petra O Nnamani; Ozioma B Onokala; Agatha A Ugwu; Adaeze L Onugwu Journal: Front Pharmacol Date: 2022-09-08 Impact factor: 5.988