S X Zheng1, L J Zhou, X Z Zhu, Y X Jin. 1. Department of Pharmacology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 200031, China.
Abstract
AIM: To study the effects of antisense vascular endothelial growth factor (VEGF) oligodeoxynucleotide (ODN) on the expression of VEGF in human glioma cell line (A172 cells). METHODS: VEGF mRNA level was measured by semiquantification reverse transcriptase polymerase chain reaction (RTPCR). VEGF protein expression in the cells was determined by immunohistochemistry. VEGF protein level in the media was measured by ELISA. RESULTS: When the cells were treated with antisense VEGF ODN (6.25-50 mumol.L-1), VEGF mRNA level in the cells decreased remarkably in a concentration-dependent manner. No change was found when the cells were treated with sense or missense ODN. When the cells were treated with antisense VEGF ODN 25 mumol.L-1, VEGF protein level decreased greatly both in the cells and the media. CONCLUSIONS: Antisense VEGF ODN inhibited VEGF expression specifically in A172 cells in vitro and thus the results provided the basis for the further studies in vivo.
AIM: To study the effects of antisense vascular endothelial growth factor (VEGF) oligodeoxynucleotide (ODN) on the expression of VEGF in humanglioma cell line (A172 cells). METHODS:VEGF mRNA level was measured by semiquantification reverse transcriptase polymerase chain reaction (RTPCR). VEGF protein expression in the cells was determined by immunohistochemistry. VEGF protein level in the media was measured by ELISA. RESULTS: When the cells were treated with antisense VEGF ODN (6.25-50 mumol.L-1), VEGF mRNA level in the cells decreased remarkably in a concentration-dependent manner. No change was found when the cells were treated with sense or missense ODN. When the cells were treated with antisense VEGF ODN 25 mumol.L-1, VEGF protein level decreased greatly both in the cells and the media. CONCLUSIONS: Antisense VEGF ODN inhibited VEGF expression specifically in A172 cells in vitro and thus the results provided the basis for the further studies in vivo.
Authors: G Cantarella; N Risuglia; R Dell'eva; L Lempereur; A Albini; G Pennisi; G M Scoto; D N Noonan; R Bernardini Journal: Br J Cancer Date: 2006-05-22 Impact factor: 7.640