| Literature DB >> 11323107 |
H Inoue1, Y Shimoyama, K Hirabayashi, H Kajigaya, S Yamamoto, H Oda, Y Koshihara.
Abstract
We examined the production of substance P (SP) in synovial fibroblasts derived from patients with rheumatoid arthritis (RA) and osteoarthritis (OA). Immunoreactive SP was observed in non-stimulated RA fibroblasts. The expression of beta-preprotachykinin-A (beta-PPT-A) mRNA was confirmed by reverse transcription-polymerase chain reaction analysis. SP contents in culture medium were increased by treatment of RA fibroblasts with transforming growth factor-beta (TGFbeta) (10 ng/ml). Levels of SP release were elevated at 12 h after TGFbeta stimulation whereas the expression of beta-PPT-A mRNA was enhanced at 3 h. Furthermore, SP production in response to TGFbeta was dose-dependently enhanced by basic fibroblast growth factor (bFGF). OA fibroblasts also significantly released SP in the presence of TGFbeta (10 ng/ml) plus bFGF (50 ng/ml). These results suggest that SP produced by synovial fibroblasts may participate in joint diseases.Entities:
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Year: 2001 PMID： 11323107 DOI： 10.1016/s0304-3940(01)01713-x
Source DB: PubMed Journal: Neurosci Lett ISSN： 0304-3940 Impact factor: 3.046