Detection of electrochemical enzymatic reactions by surface plasmon resonance measurement.

We describe the surface plasmon resonance (SPR) detection of an enzymatic turnover reaction and the measurement of glucose concentration using a multienzyme layer modified gold electrode. We constructed an osmium redox polymer mediated enzyme sensor on a gold thin-film electrode and monitored electrochemical reaction by SPR measurement. Unlike the usual binding assay with SPR, here we used SPR to detect the redox state of an electron mediator that was the result of the electron-transfer reaction of sequential enzymatic reactions. Therefore, the degree of refractive index change was independent of the dielectric property of the substrate and enzymatic molecular recognition was converted to refractive index change with amplification. For the quantitative evaluation of glucose with this method, we used chronopotentiometry and a linear relation was obtained between the glucose concentration and the rate of refractive index change.