AIMS/ BACKGROUND: The hepatitis C virus (HCV) core protein is known to play an important role in hepatocarcinogenesis. Recent studies have suggested that the increased proliferation rate of hepatocytes is a major risk factor for the development of hepatocellular carcinoma. In this study, we investigated whether the HCV core protein promotes the cell growth rate through the modulation of cyclin E expression levels. METHODS/ RESULTS: HCV core stable transfectant Rat-1 cell lines showed a markedly increased proliferation rate compared to mock cells. Cyclin E expression and its associated kinase activities were remarkably increased in HCV core stable transfectants. Cyclin E mRNA levels were also upregulated in these cell lines. CONCLUSIONS: Our data suggest that the HCV core protein promotes cell proliferation through upregulation of the cyclin E expression levels, implying this property of HCV core protein plays an important role in hepatocarcinogenesis.
AIMS/ BACKGROUND: The hepatitis C virus (HCV) core protein is known to play an important role in hepatocarcinogenesis. Recent studies have suggested that the increased proliferation rate of hepatocytes is a major risk factor for the development of hepatocellular carcinoma. In this study, we investigated whether the HCV core protein promotes the cell growth rate through the modulation of cyclin E expression levels. METHODS/ RESULTS:HCV core stable transfectant Rat-1 cell lines showed a markedly increased proliferation rate compared to mock cells. Cyclin E expression and its associated kinase activities were remarkably increased in HCV core stable transfectants. Cyclin E mRNA levels were also upregulated in these cell lines. CONCLUSIONS: Our data suggest that the HCV core protein promotes cell proliferation through upregulation of the cyclin E expression levels, implying this property of HCV core protein plays an important role in hepatocarcinogenesis.
Authors: Ke Li; Lin Wang; Jun Cheng; Yin-Ying Lu; Ling-Xin Zhang; Jin-Song Mu; Yuan Hong; Yan Liu; Hui-Juan Duan; Gang Wang; Li Li; Ju-Mei Chen Journal: World J Gastroenterol Date: 2003-02 Impact factor: 5.742
Authors: Sarah L Fishman; Stephanie H Factor; Cinzia Balestrieri; Xiaofeng Fan; Adrian M Dibisceglie; Suresh M Desai; Gary Benson; Andrea D Branch Journal: Clin Cancer Res Date: 2009-04-21 Impact factor: 12.531
Authors: Yan Geng; Wojciech Michowski; Joel M Chick; Yaoyu E Wang; M Emmanuelle Jecrois; Katharine E Sweeney; Lijun Liu; Richard C Han; Nan Ke; Agnieszka Zagozdzon; Ewa Sicinska; Roderick T Bronson; Steven P Gygi; Piotr Sicinski Journal: Proc Natl Acad Sci U S A Date: 2018-01-16 Impact factor: 11.205