R Talwar1, L Di Silvio, F J Hughes, G N King. 1. Department of Periodontology, St Bartholomew's and the Royal London School of Medicine and Dentistry, University of London, UK.
Abstract
BACKGROUND: Bone morphogenetic proteins (BMPs) have shown considerable promise as a therapeutic agent to enhance periodontal regeneration although the optimal characteristics of a suitable release system are not known. AIM: The aim of this study was to compare the effects of slow and fast degrading gelatin carriers on BMP-2-induced periodontal healing. METHOD: Recombinant human bone morphogenetic protein-2 (rhBMP-2) was incorporated into gelatin and subsequently differentially cross-linked to produce slow and fast release carrier systems. Release kinetics were confirmed in vitro, by measuring release of 125I-growth hormone from similar gelatin plugs. Effects of BMP were evaluated in surgically created rat periodontal fenestration defects which were processed for histology 10 days post-operatively. The rats were divided into 4 groups and the control defects were treated with either slow or fast degrading gelatin (CONs or CONf respectively), whilst test groups were treated with 1.25 microg rhBMP-2 in the slow or fast degrading gelatin (BMPs or BMPf respectively). RESULTS: BMPf greatly increased bone formation compared with the control (CONf) (1.67 +/- 0.65 versus 0.34 +/- 0.11 x 10(-4) m2), but no significant differences were observed with BMPs and CONs. In contrast, new cementum formation was significantly greater in the BMPs group compared with all other groups (p<0.05). CONCLUSION: Release kinetics of BMP may have important effects on the outcome of BMP-induced periodontal regeneration. New bone formation may be affected by rapid-release kinetics although further investigation is necessary to confirm this. In contrast, new cementum formation is promoted by slow release of BMP.
BACKGROUND: Bone morphogenetic proteins (BMPs) have shown considerable promise as a therapeutic agent to enhance periodontal regeneration although the optimal characteristics of a suitable release system are not known. AIM: The aim of this study was to compare the effects of slow and fast degrading gelatin carriers on BMP-2-induced periodontal healing. METHOD: Recombinant humanbone morphogenetic protein-2 (rhBMP-2) was incorporated into gelatin and subsequently differentially cross-linked to produce slow and fast release carrier systems. Release kinetics were confirmed in vitro, by measuring release of 125I-growth hormone from similar gelatin plugs. Effects of BMP were evaluated in surgically created rat periodontal fenestration defects which were processed for histology 10 days post-operatively. The rats were divided into 4 groups and the control defects were treated with either slow or fast degrading gelatin (CONs or CONf respectively), whilst test groups were treated with 1.25 microg rhBMP-2 in the slow or fast degrading gelatin (BMPs or BMPf respectively). RESULTS:BMPf greatly increased bone formation compared with the control (CONf) (1.67 +/- 0.65 versus 0.34 +/- 0.11 x 10(-4) m2), but no significant differences were observed with BMPs and CONs. In contrast, new cementum formation was significantly greater in the BMPs group compared with all other groups (p<0.05). CONCLUSION: Release kinetics of BMP may have important effects on the outcome of BMP-induced periodontal regeneration. New bone formation may be affected by rapid-release kinetics although further investigation is necessary to confirm this. In contrast, new cementum formation is promoted by slow release of BMP.
Authors: Subha N Rath; Galyna Pryymachuk; Oliver A Bleiziffer; Christopher X F Lam; Andreas Arkudas; Saey T B Ho; Justus P Beier; Raymund E Horch; Dietmar W Hutmacher; Ulrich Kneser Journal: J Mater Sci Mater Med Date: 2011-03-30 Impact factor: 3.896
Authors: Oliver B Betz; Volker M Betz; Christian Schröder; Rainer Penzkofer; Michael Göttlinger; Susanne Mayer-Wagner; Peter Augat; Volkmar Jansson; Peter E Müller Journal: BMC Biotechnol Date: 2013-08-08 Impact factor: 2.563