| Literature DB >> 11313784 |
M Tanaka1, K Kogawa, K Nakamura, Y Nishihori, K Kuribayashi, S Hagiwara, H Muramatsu, S Sakamaki, Y Niitsu.
Abstract
We have previously reported that superoxide stimulates the motility of tumor cells and the administration of Cu-Zn superoxide dismutase (SOD) significantly suppresses metastasis. However, ideally, anti-metastatic therapy should be long-lasting, systemically effective and have low toxicity. The half-life of Cu-Zn SOD in plasma is so short that it cannot provide long-lasting effects. Therefore, in this study we have developed a gene therapy in a mouse model utilizing extracellular SOD (EC-SOD), which is the most prevalent SOD isoenzyme in extracellular fluids. We retrovirally transfected fibroblasts (syngeneic) with the EC-SOD gene and established EC-SOD-secreting fibroblasts. Inoculation of EC-SOD-secreting fibroblasts suppressed both artificial and spontaneous metastatic lung nodules in mouse metastasis models. These data indicate the feasibility of anti-metastatic gene therapy utilizing the EC-SOD gene.Entities:
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Year: 2001 PMID: 11313784 DOI: 10.1038/sj.gt.3301362
Source DB: PubMed Journal: Gene Ther ISSN: 0969-7128 Impact factor: 5.250