Literature DB >> 11313784

Anti-metastatic gene therapy utilizing subcutaneous inoculation of EC-SOD gene transduced autologous fibroblast suppressed lung metastasis of Meth-A cells and 3LL cells in mice.

M Tanaka1, K Kogawa, K Nakamura, Y Nishihori, K Kuribayashi, S Hagiwara, H Muramatsu, S Sakamaki, Y Niitsu.   

Abstract

We have previously reported that superoxide stimulates the motility of tumor cells and the administration of Cu-Zn superoxide dismutase (SOD) significantly suppresses metastasis. However, ideally, anti-metastatic therapy should be long-lasting, systemically effective and have low toxicity. The half-life of Cu-Zn SOD in plasma is so short that it cannot provide long-lasting effects. Therefore, in this study we have developed a gene therapy in a mouse model utilizing extracellular SOD (EC-SOD), which is the most prevalent SOD isoenzyme in extracellular fluids. We retrovirally transfected fibroblasts (syngeneic) with the EC-SOD gene and established EC-SOD-secreting fibroblasts. Inoculation of EC-SOD-secreting fibroblasts suppressed both artificial and spontaneous metastatic lung nodules in mouse metastasis models. These data indicate the feasibility of anti-metastatic gene therapy utilizing the EC-SOD gene.

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Year:  2001        PMID: 11313784     DOI: 10.1038/sj.gt.3301362

Source DB:  PubMed          Journal:  Gene Ther        ISSN: 0969-7128            Impact factor:   5.250


  6 in total

1.  Modulation of redox signaling promotes apoptosis in epithelial ovarian cancer cells.

Authors:  Zhongliang Jiang; Nicole M Fletcher; Rouba Ali-Fehmi; Michael P Diamond; Husam M Abu-Soud; Adnan R Munkarah; Ghassan M Saed
Journal:  Gynecol Oncol       Date:  2011-05-26       Impact factor: 5.482

2.  Optimizing an Antioxidant TEMPO Copolymer for Reactive Oxygen Species Scavenging and Anti-Inflammatory Effects in Vivo.

Authors:  Carlisle R DeJulius; Bryan R Dollinger; Taylor E Kavanaugh; Eric Dailing; Fang Yu; Shubham Gulati; Angelo Miskalis; Caiyun Zhang; Jashim Uddin; Sergey Dikalov; Craig L Duvall
Journal:  Bioconjug Chem       Date:  2021-04-19       Impact factor: 4.774

3.  Essential role of protein kinase C zeta in transducing a motility signal induced by superoxide and a chemotactic peptide, fMLP.

Authors:  Kageaki Kuribayashi; Kiminori Nakamura; Maki Tanaka; Tsutomu Sato; Junji Kato; Katsunori Sasaki; Rishu Takimoto; Katsuhisa Kogawa; Takeshi Terui; Tetsuji Takayama; Takayuki Onuma; Takuya Matsunaga; Yoshiro Niitsu
Journal:  J Cell Biol       Date:  2007-03-26       Impact factor: 10.539

4.  Prevention of inflammation-mediated acquisition of metastatic properties of benign mouse fibrosarcoma cells by administration of an orally available superoxide dismutase.

Authors:  F Okada; H Shionoya; M Kobayashi; T Kobayashi; H Tazawa; K Onuma; Y Iuchi; N Matsubara; T Ijichi; B Dugas; M Hosokawa
Journal:  Br J Cancer       Date:  2006-03-27       Impact factor: 7.640

5.  Extracellular superoxide dismutase regulates the expression of small gtpase regulatory proteins GEFs, GAPs, and GDI.

Authors:  Mikko O Laukkanen; Francesca Cammarota; Tiziana Esposito; Marco Salvatore; Maria D Castellone
Journal:  PLoS One       Date:  2015-03-09       Impact factor: 3.240

Review 6.  Extracellular Superoxide Dismutase: Growth Promoter or Tumor Suppressor?

Authors:  Mikko O Laukkanen
Journal:  Oxid Med Cell Longev       Date:  2016-05-12       Impact factor: 6.543

  6 in total

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