OBJECTIVES: Peptidoglycan (PG), a component of Gram-positive bacteria, may be involved in rheumatoid arthritis (RA) because of its ability to induce production of proinflammatory cytokines, to induce arthritis in rodents, and its presence in antigen-presenting cells in RA joints. METHODS: In the present study, physiologically relevant PG was able to induce T-cell proliferation in peripheral blood and synovial fluid samples of RA patients, but the magnitude of the response did not differ from that of cells from healthy subjects. In addition, production of cytokines associated with RA (interleukins (IL)-1beta, IL-6, IL-8, IL-10, IL-12 and tumour necrosis factor alpha) and of the matrix metalloproteinase, gelatinase B (MMP-9), was induced in blood and synovial fluid cultures of RA patients. CONCLUSION: The fact that PG, which can be found in synovial tissues of RA patients is able to induce the production of inflammatory mediators supports the hypothesis that PG plays a role in the pathogenesis of RA by influencing the inflammatory microenvironment of the joint.
OBJECTIVES: Peptidoglycan (PG), a component of Gram-positive bacteria, may be involved in rheumatoid arthritis (RA) because of its ability to induce production of proinflammatory cytokines, to induce arthritis in rodents, and its presence in antigen-presenting cells in RA joints. METHODS: In the present study, physiologically relevant PG was able to induce T-cell proliferation in peripheral blood and synovial fluid samples of RApatients, but the magnitude of the response did not differ from that of cells from healthy subjects. In addition, production of cytokines associated with RA (interleukins (IL)-1beta, IL-6, IL-8, IL-10, IL-12 and tumour necrosis factor alpha) and of the matrix metalloproteinase, gelatinase B (MMP-9), was induced in blood and synovial fluid cultures of RApatients. CONCLUSION: The fact that PG, which can be found in synovial tissues of RApatients is able to induce the production of inflammatory mediators supports the hypothesis that PG plays a role in the pathogenesis of RA by influencing the inflammatory microenvironment of the joint.
Authors: Lizette Visser; Marie-José Melief; Debby van Riel; Marjan van Meurs; Ella A Sick; Seiichi Inamura; Jeffrey J Bajramovic; Sandra Amor; Rogier Q Hintzen; Leonie A Boven; Bert A 't Hart; Jon D Laman Journal: Am J Pathol Date: 2006-11 Impact factor: 4.307
Authors: Payal P Balraadjsing; Lisbeth D Lund; Yuri Souwer; Sebastian A J Zaat; Hanne Frøkiær; Esther C de Jong Journal: Infect Immun Date: 2019-12-17 Impact factor: 3.441
Authors: Brandon L Jutras; Robert B Lochhead; Zachary A Kloos; Jacob Biboy; Klemen Strle; Carmen J Booth; Sander K Govers; Joe Gray; Peter Schumann; Waldemar Vollmer; Linda K Bockenstedt; Allen C Steere; Christine Jacobs-Wagner Journal: Proc Natl Acad Sci U S A Date: 2019-06-17 Impact factor: 11.205
Authors: Jason R Bobe; Brandon L Jutras; Elizabeth J Horn; Monica E Embers; Allison Bailey; Robert L Moritz; Ying Zhang; Mark J Soloski; Richard S Ostfeld; Richard T Marconi; John Aucott; Avi Ma'ayan; Felicia Keesing; Kim Lewis; Choukri Ben Mamoun; Alison W Rebman; Mecaila E McClune; Edward B Breitschwerdt; Panga Jaipal Reddy; Ricardo Maggi; Frank Yang; Bennett Nemser; Aydogan Ozcan; Omai Garner; Dino Di Carlo; Zachary Ballard; Hyou-Arm Joung; Albert Garcia-Romeu; Roland R Griffiths; Nicole Baumgarth; Brian A Fallon Journal: Front Med (Lausanne) Date: 2021-08-18