Literature DB >> 11311150

Characterization of membrane-localized and cytosolic Rac-GTPase-activating proteins in human neutrophil granulocytes: contribution to the regulation of NADPH oxidase.

M Geiszt1, M C Dagher, G Molnár, A Havasi, J Faure, M H Paclet, F Morel, E Ligeti.   

Abstract

We have investigated the intracellular localization and molecular identity of Rac-GTPase-activating proteins (Rac-GAPs) in human neutrophils. Immunoblot analysis detected the presence of both p190RhoGAP and Bcr mainly in the cytosol. An overlay assay performed with [gamma-(32)P]GTP-bound Rac revealed dominant GAP activity related to a 50 kDa protein both in the membrane and cytosol. This activity could be identified by Western blotting and immunoprecipitation with specific antibody directed against the GAP domain of p50RhoGAP. Using a semirecombinant or fully purified cell-free activation assay of the Rac-activated enzyme NADPH oxidase, we demonstrated the regulatory effect of both the membrane-localized and soluble GAPs. We suggest that in neutrophil granulocytes Rac-GAPs have redundant function and represent suitable targets for both the up-regulation and down-regulation of the NADPH oxidase.

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Year:  2001        PMID： 11311150      PMCID： PMC1221803          DOI： 10.1042/bj3550851

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  54 in total

1.  Activation of the NADPH oxidase involves the small GTP-binding protein p21rac1.

Authors:  A Abo; E Pick; A Hall; N Totty; C G Teahan; A W Segal
Journal:  Nature       Date:  1991-10-17       Impact factor: 49.962

Review 2.  The biochemical basis of the NADPH oxidase of phagocytes.

Authors:  A W Segal; A Abo
Journal:  Trends Biochem Sci       Date:  1993-02       Impact factor: 13.807

3.  An essential role for Rac in Ras transformation.

Authors:  R G Qiu; J Chen; D Kirn; F McCormick; M Symons
Journal:  Nature       Date:  1995-03-30       Impact factor: 49.962

4.  Wortmannin is a potent phosphatidylinositol 3-kinase inhibitor: the role of phosphatidylinositol 3,4,5-trisphosphate in neutrophil responses.

Authors:  A Arcaro; M P Wymann
Journal:  Biochem J       Date:  1993-12-01       Impact factor: 3.857

5.  Cloning and expression of a human CDC42 GTPase-activating protein reveals a functional SH3-binding domain.

Authors:  E T Barfod; Y Zheng; W J Kuang; M J Hart; T Evans; R A Cerione; A Ashkenazi
Journal:  J Biol Chem       Date:  1993-12-15       Impact factor: 5.157

6.  Germ cell beta-chimaerin, a new GTPase-activating protein for p21rac, is specifically expressed during the acrosomal assembly stage in rat testis.

Authors:  T Leung; B E How; E Manser; L Lim
Journal:  J Biol Chem       Date:  1993-02-25       Impact factor: 5.157

7.  Selective activation of the JNK signaling cascade and c-Jun transcriptional activity by the small GTPases Rac and Cdc42Hs.

Authors:  A Minden; A Lin; F X Claret; A Abo; M Karin
Journal:  Cell       Date:  1995-06-30       Impact factor: 41.582

8.  Increased neutrophil respiratory burst in bcr-null mutants.

Authors:  J W Voncken; H van Schaick; V Kaartinen; K Deemer; T Coates; B Landing; P Pattengale; O Dorseuil; G M Bokoch; J Groffen
Journal:  Cell       Date:  1995-03-10       Impact factor: 41.582

9.  Activation of the O2(-)-generating NADPH oxidase in a semi-recombinant cell-free system. Assessment of the function of Rac in the activation process.

Authors:  A Fuchs; M C Dagher; A Jouan; P V Vignais
Journal:  Eur J Biochem       Date:  1994-12-01

10.  A novel role for RhoGDI as an inhibitor of GAP proteins.

Authors:  J F Hancock; A Hall
Journal:  EMBO J       Date:  1993-05       Impact factor: 11.598
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  7 in total

1.  Rho/RacGAPs: embarras de richesse?

Authors:  Roland Csépányi-Kömi; Magdolna Lévay; Erzsébet Ligeti
Journal:  Small GTPases       Date:  2012-07-01

2.  Role of nucleotides and phosphoinositides in the stability of electron and proton currents associated with the phagocytic NADPH oxidase.

Authors:  Gábor L Petheo; Nathalie C Girardin; Nicolas Goossens; Gergely Z Molnár; Nicolas Demaurex
Journal:  Biochem J       Date:  2006-12-15       Impact factor: 3.857

Review 3.  Role of the Rho GTPase Rac in the activation of the phagocyte NADPH oxidase: outsourcing a key task.

Authors:  Edgar Pick
Journal:  Small GTPases       Date:  2014-03-05

4.  Neutrophil functions and autoimmune arthritis in the absence of p190RhoGAP: generation and analysis of a novel null mutation in mice.

Authors:  Tamás Németh; Krisztina Futosi; Csilla Hably; Madeleine R Brouns; Sascha M Jakob; Miklós Kovács; Zsuzsanna Kertész; Barbara Walzog; Jeffrey Settleman; Attila Mócsai
Journal:  J Immunol       Date:  2010-07-30       Impact factor: 5.422

5.  Ehrlichia chaffeensis Uses an Invasin To Suppress Reactive Oxygen Species Generation by Macrophages via CD147-Dependent Inhibition of Vav1 To Block Rac1 Activation.

Authors:  Omid Teymournejad; Yasuko Rikihisa
Journal:  mBio       Date:  2020-04-21       Impact factor: 7.867

Review 6.  The "Biological Weapons" of Ehrlichia chaffeensis: Novel Molecules and Mechanisms to Subjugate Host Cells.

Authors:  Yasuko Rikihisa
Journal:  Front Cell Infect Microbiol       Date:  2022-01-14       Impact factor: 5.293

7.  Computational analysis of Rho GTPase cycling.

Authors:  Cibele Vieira Falkenberg; Leslie M Loew
Journal:  PLoS Comput Biol       Date:  2013-01-10       Impact factor: 4.475
  7 in total

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