| Literature DB >> 11306110 |
S M Tanhauser1, M A Cheadle, E T Massey, B A Mayer, D E Schroedter, J B Dame, E C Greiner, R J MacKay.
Abstract
Sarcocysts were dissected from the tongue of a nine-banded armadillo (Dasypus novemcinctus). DNA was extracted and characterised by PCR amplification followed by restriction fragment length polymorphism analysis and nucleotide sequencing. A total of 1879 nucleotides were compared; the sarcocyst DNA sequence was identical to that reported for Sarcocystis neurona. DNA was extracted from the sarcocysts of five more nine-banded armadillos. A 254-nucleotide sequence was determined for each and found to be identical to S. neurona. Western blot techniques for detection of anti-S. neurona antibody were developed for use with armadillo plasma and samples from 19 wild-caught and 17 captive-raised armadillos were examined. Whereas all of the 19 wild-caught armadillos had antibodies to S. neurona, only one of 17 captive-raised armadillos did. These results suggest that the nine-banded armadillo are naturally infected with S. neurona.Entities:
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Year: 2001 PMID: 11306110 DOI: 10.1016/s0020-7519(01)00178-3
Source DB: PubMed Journal: Int J Parasitol ISSN: 0020-7519 Impact factor: 3.981