| Literature DB >> 11303561 |
E V Bichenkova1, S Frau, O S Fedorova, K T Douglas.
Abstract
The binding of the conjugate of Hoechst 33258 with 5,10,15,20-tetrakis (1-methyl-4-pyridyl)-21H,23H-porphyrin (PORHOE) to single-strand DNA has been detected by UV-vis spectrophotometry and 1H-NMR. The red-shift of porphyrin Soret band with strong hypochromicity indicates that the porphyrin moiety dominates in the interaction of the PORHOE with ssDNA. The affinity constants of PORHOE for d(GCATACAATTCG) or d(CGAATTGTATGC) were determined to be >10(5) M(-1), with strong cooperativity.Entities:
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Year: 2001 PMID： 11303561 DOI： 10.1081/NCN-100001444
Source DB: PubMed Journal: Nucleosides Nucleotides Nucleic Acids ISSN： 1525-7770 Impact factor: 1.381