Literature DB >> 1129141

Preparation and separation of d(pT)-10-n oligonucleotides.

A J Raae, J R Lillehaug, R K Kleppe, K Kleppe.   

Abstract

A series of oligomers having the general formula d(pT)-10-n, n varying from 2 to 20, has been prepared by enzymatic joining of d(pT)-10, annealed on poly dA, employing T-4 polynucleotide ligase. The oligomers could be separated on 8 or 12% polyacrylamide gels. Such oligomers may prove useful as molecular weight markers and initiators for various polymerases.

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Year:  1975        PMID： 1129141      PMCID： PMC342847          DOI： 10.1093/nar/2.3.423

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  8 in total

1.  Persistence of deoxyribonucleic acid polymerase I and its 5'--3' exonuclease activity in PolA mutants of Escherichia coli K12.

Authors:  I R Lehman; J R Chien
Journal:  J Biol Chem       Date:  1973-11-25       Impact factor: 5.157

2.  Physical characterization and simultaneous purification of bacteriophage T4 induced polynucleotide kinase, polynucleotide ligase, and deoxyribonucleic acid polymerase.

Authors:  A Panet; J H van de Sande; P C Loewen; H G Khorana; A J Raae; J R Lillehaug; K Kleppe
Journal:  Biochemistry       Date:  1973-12-04       Impact factor: 3.162

3.  Synthetic DNA-RNA hybrids and RNA-RNA duplexes as templates for the polymerases of the oncogenic RNA viruses.

Authors:  S Spiegelman; A Burny; M R Das; J Keydar; J Schlom; M Trávnícek; K Watson
Journal:  Nature       Date:  1970-10-31       Impact factor: 49.962

4.  DNA polymerase I from Acinetobacter calcoaceticus: enzymatic properties.

Authors:  I F Nes; J R Lillehaug; K Kleppe
Journal:  Eur J Biochem       Date:  1974-12-16

5.  The enzymatic phosphorylation of ribonucleic acid and deoxyribonucleic acid. II. Further properties of the 5'-hydroxyl polynucleotide kinase.

Authors:  A Novogrodsky; M Tal; A Traub; J Hurwitz
Journal:  J Biol Chem       Date:  1966-06-25       Impact factor: 5.157

6.  Synthesis and characterization of the duplex block polymer d(C15A15)-d(T15G15).

Authors:  J F Burd; R D Wells
Journal:  J Biol Chem       Date:  1974-11-25       Impact factor: 5.157

7.  Studies on polynucleotides. LXXXVII. The joining of short deoxyribopolynucleotides by DNA-joining enzymes.

Authors:  N K Gupta; E Ohtsuka; H Weber; S H Chang; H G Khorana
Journal:  Proc Natl Acad Sci U S A       Date:  1968-05       Impact factor: 11.205

8.  A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity.

Authors:  I M Glynn; J B Chappell
Journal:  Biochem J       Date:  1964-01       Impact factor: 3.857
  8 in total
  1 in total

1.  Characterization of in vitro transcription initiation and termination sites in Col E1 DNA.

Authors:  R K Patient
Journal:  Nucleic Acids Res       Date:  1979-06-25       Impact factor: 16.971
  1 in total

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