Literature DB >> 11286334

Operational characteristics of an antibody-immobilized QCM system detecting Salmonella spp.

I S Park1, W Y Kim, N Kim.   

Abstract

A quartz crystal microbalance (QCM) system detecting Salmonella spp. was developed by an anti-Salmonella antibody immobilization onto one gold surface of a piezoelectric quartz crystal surface with sulfosuccinimidyl 6-[3-(2-pyridyldithio)propionamido]hexanoate (sulfo-LC-SPDP) thiolation. The optimum temperature and pH for the antibody-immobilized sensor were 35 degrees C and 7.2, respectively. The frequency shifts obtained were correlated with the Salmonella concentrations in the range 3.2 x 10(6)-4.8 x 10(8) CFU per ml. The system was quite specific to Salmonella spp. and applicable for repetitive use after a regeneration step employing 1.2 M NaOH. A model sample measurement was done for a market milk spiked with Salmonella typhimurium.

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Year:  2000        PMID： 11286334     DOI： 10.1016/s0956-5663(00)00053-1

Source DB:  PubMed          Journal:  Biosens Bioelectron        ISSN： 0956-5663            Impact factor:   10.618


  6 in total

1.  A Novel Prototype Biosensor Array Electrode System for Detecting the Bacterial Pathogen Salmonella typhimurium.

Authors:  Palaniappan Ramasamy; Gajalakshmi Dakshinamoorthy; Shanmugam Jayashree; Dhamodharan Prabhu; Sundararaj Rajamanikandan; Palaniyandi Velusamy; Govindan Dayanithi; Robert E B Hanna
Journal:  Biosensors (Basel)       Date:  2022-06-04

2.  AFM Specific Identification of Bacterial Cell Fragments on Biofunctional Surfaces.

Authors:  Evgeniy V Dubrovin; Galina N Fedyukina; Sergey V Kraevsky; Tatiana E Ignatyuk; Igor V Yaminsky; Sergei G Ignatov
Journal:  Open Microbiol J       Date:  2012-02-23

3.  A label-free immunosensor for ultrasensitive detection of ketamine based on quartz crystal microbalance.

Authors:  Ya Yang; Yifeng Tu; Xiaoshu Wang; Jinyin Pan; Yun Ding
Journal:  Sensors (Basel)       Date:  2015-04-13       Impact factor: 3.576

4.  Early detection of bacteria using SPR imaging and event counting: experiments with Listeria monocytogenes and Listeria innocua.

Authors:  Marine Boulade; Alexandra Morlay; Felix Piat; Yoann Roupioz; Thierry Livache; Paul G Charette; Michael Canva; Loïc Leroy
Journal:  RSC Adv       Date:  2019-05-17       Impact factor: 4.036

Review 5.  Bulk and Surface Acoustic Wave Biosensors for Milk Analysis.

Authors:  Kerstin Länge
Journal:  Biosensors (Basel)       Date:  2022-08-05

6.  The detection of influenza A and B viruses in clinical specimens using a quartz crystal microbalance.

Authors:  Thamara M Peduru Hewa; Gregory A Tannock; David E Mainwaring; Sally Harrison; John V Fecondo
Journal:  J Virol Methods       Date:  2009-07-21       Impact factor: 2.014
  6 in total

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