OBJECTIVE: To detect lymphocyte subpopulations and CD3+/DR + expression in sepsis. METHODS: In a prospective clinical study we evaluated subpopulations of lymphocytes and percentage of CD3+/HLA-DR+ lymphocytes using two-color flow cytometry in 40 patients with sepsis and compared them with 34 healthy adults. RESULTS: Septic patients, when compared with healthy controls, have significantly lower percentage and absolute numbers of total T lymphocytes and CD4 T lymphocytes (P < 0.01). Absolute numbers of CD8 T lymphocytes, NK cells, CD3+/DR + lymphocytes and CD4/CD8 ratio were also decreased (P < 0.01). The percentage of B lymphocytes was increased (P < 0.01). CONCLUSION: Our results are in agreement with previous findings in patients with sepsis after major surgery or trauma. The decreases in the percentage and absolute numbers of circulating lymphocyte subsets in non-surgical sepsis could represent a general reaction to stress. Increased percentage of B lymphocytes is most probably related to the bacterial etiology of the disease.
OBJECTIVE: To detect lymphocyte subpopulations and CD3+/DR + expression in sepsis. METHODS: In a prospective clinical study we evaluated subpopulations of lymphocytes and percentage of CD3+/HLA-DR+ lymphocytes using two-color flow cytometry in 40 patients with sepsis and compared them with 34 healthy adults. RESULTS: Septic patients, when compared with healthy controls, have significantly lower percentage and absolute numbers of total T lymphocytes and CD4 T lymphocytes (P < 0.01). Absolute numbers of CD8 T lymphocytes, NK cells, CD3+/DR + lymphocytes and CD4/CD8 ratio were also decreased (P < 0.01). The percentage of B lymphocytes was increased (P < 0.01). CONCLUSION: Our results are in agreement with previous findings in patients with sepsis after major surgery or trauma. The decreases in the percentage and absolute numbers of circulating lymphocyte subsets in non-surgical sepsis could represent a general reaction to stress. Increased percentage of B lymphocytes is most probably related to the bacterial etiology of the disease.
Authors: A Denizmen Aygun; A Nese Citak Kurt; Ahmet Godekmerdan; Abdullah Kurt; Saadet Akarsu; M Kaya Gurgoze; Erdal Yilmaz Journal: Inflammation Date: 2008-05-01 Impact factor: 4.092
Authors: Jorge Monserrat; Raul de Pablo; Eduardo Reyes; David Díaz; Hugo Barcenilla; Manuel R Zapata; Antonio De la Hera; Alfredo Prieto; Melchor Alvarez-Mon Journal: Crit Care Date: 2009-02-25 Impact factor: 9.097
Authors: L Vanderbeke; P Van Mol; Y Van Herck; F De Smet; S Humblet-Baron; K Martinod; A Antoranz; I Arijs; B Boeckx; F M Bosisio; M Casaer; D Dauwe; W De Wever; C Dooms; E Dreesen; A Emmaneel; J Filtjens; M Gouwy; J Gunst; G Hermans; S Jansen; K Lagrou; A Liston; N Lorent; P Meersseman; T Mercier; J Neyts; J Odent; D Panovska; P A Penttila; E Pollet; P Proost; J Qian; K Quintelier; J Raes; S Rex; Y Saeys; J Sprooten; S Tejpar; D Testelmans; K Thevissen; T Van Buyten; J Vandenhaute; S Van Gassen; L C Velásquez Pereira; R Vos; B Weynand; A Wilmer; J Yserbyt; A D Garg; P Matthys; C Wouters; D Lambrechts; E Wauters; J Wauters Journal: Nat Commun Date: 2021-07-05 Impact factor: 14.919
Authors: Tatiana P da Silva; Carmem B W Giacoia-Gripp; Carolina A Schmaltz; Flavia M Sant Anna; Valeria Rolla; Mariza G Morgado Journal: PLoS One Date: 2013-06-19 Impact factor: 3.240