Literature DB >> 11283101

Random amplified polymorphic DNA assay as a rapid tool in screening for Neisseria meningitidis serogroup C isolates of electrophoretic type 24.

S Schmink1, M W Reeves, B Plikaytis, T Popovic.   

Abstract

Neisseria meningitidis serogroup C (NMSC) isolates of electrophoretic type 24 (ET-24), as identified by multilocus enzyme electrophoresis, are the main cause of serogroup C meningococcal disease outbreaks and sporadic meningococcal disease in the United States. We evaluated a random amplified polymorphic DNA assay as a rapid tool to screen for isolates of ET-24 by testing 199 NMSC isolates of 51 different ETs. A sensitivity of 88% and a specificity of 87% was achieved in identification of ET-24 isolates when the patterns obtained by two primers, P1 and P5, were analyzed together.

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Year:  2001        PMID： 11283101      PMCID： PMC87984          DOI： 10.1128/JCM.39.4.1622-1625.2001

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  4 in total

Review 1.  Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics.

Authors:  R K Selander; D A Caugant; H Ochman; J M Musser; M N Gilmour; T S Whittam
Journal:  Appl Environ Microbiol       Date:  1986-05       Impact factor: 4.792

2.  Use of arbitrarily primed polymerase chain reaction analysis to type disease and carrier strains of Neisseria meningitidis isolated during a university outbreak.

Authors:  J P Woods; D Kersulyte; R W Tolan; C M Berg; D E Berg
Journal:  J Infect Dis       Date:  1994-06       Impact factor: 5.226

3.  Evaluation of pulsed-field gel electrophoresis in epidemiological investigations of meningococcal disease outbreaks caused by Neisseria meningitidis serogroup C.

Authors:  T Popovic; S Schmink; N A Rosenstein; G W Ajello; M W Reeves; B Plikaytis; S B Hunter; E M Ribot; D Boxrud; M L Tondella; C Kim; C Noble; E Mothershed; J Besser; B A Perkins
Journal:  J Clin Microbiol       Date:  2001-01       Impact factor: 5.948

4.  Randomly amplified polymorphic DNA genotyping of serogroup A meningococci yields results similar to those obtained by multilocus enzyme electrophoresis and reveals new genotypes.

Authors:  A Bart; I G Schuurman; M Achtman; D A Caugant; J Dankert; A van der Ende
Journal:  J Clin Microbiol       Date:  1998-06       Impact factor: 5.948
  4 in total

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