| Literature DB >> 11282401 |
N V Strashnikova1, N Medvedeva, G I Likhtenshtein.
Abstract
A novel method has been developed for measuring depth of immersion of a fluorescent chromophore in biological matrices, such as biomembranes. The method is based on dynamic quenching of chromophore fluorescence by a nitroxide probe freely diffused in solution. Theoretical considerations and experimental evidences relating to the method are discussed. The proposed method was applied to investigation of lecithin liposomes and membranes from Bacillus subtilis modified by the photochrome-fluorescent probe 4,4'-dimethylaminocyanostilbene.Entities:
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Year: 2001 PMID： 11282401 DOI： 10.1016/s0165-022x(00)00144-5
Source DB: PubMed Journal: J Biochem Biophys Methods ISSN： 0165-022X