Use of internal vial for determining quench correction in scintillation counting of heterogeneous media.

An insert vial situated at the center of the counting medium and containing a standard count has been used for quench correction in the liquid scintillation counting of gels containing suspensions of silicic acid scrapings from thin layer chromatoplates. The method has provided a simple and direct means of routine count correction for a range of 14-C-labeled lipid classes following separation on thin layer chromatoplates. The use of insert vial provides several advantages over other methods for the count correction of these suspensions.