| Literature DB >> 11277915 |
E Clynen1, G Baggerman, D Veelaert, A Cerstiaens, D Van der Horst, L Harthoorn, R Derua, E Waelkens, A De Loof, L Schoofs.
Abstract
The pars intercerebralis-corpora cardiaca system (PI-CC) of insects is the endocrinological equivalent of the hypothalamus-pituitary system of vertebrates. Peptide profiles of the pars intercerebralis and the corpora cardiaca were characterized using simple sampling protocols in combination with MALDI-TOF and electrospray ionization double quadrupole time of flight (ESI-Qq-TOF) mass spectrometric technologies. The results were compared with earlier results of conventional sequencing methods and immunocytochemical methods. In addition to many known peptides, several m/z signals corresponding to putative novel peptides were observed in the corpora cardiaca and/or pars intercerebralis. Furthermore, for a number of peptides evidence was provided about their localization and MALDI-TOF analysis of the released material from the corpora cardiaca yielded information on the hormonal status of particular brain peptides.Entities:
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Year: 2001 PMID： 11277915 DOI： 10.1046/j.1432-1327.2001.02067.x
Source DB: PubMed Journal: Eur J Biochem ISSN： 0014-2956