Literature DB >> 11277442

Stable RK2-derived cloning vectors for the analysis of gene expression and gene function in gram-negative bacteria.

B Dombrecht1, J Vanderleyden, J Michiels.   

Abstract

The construction of several stable RK2-derived cloning vectors for the analysis of gene expression and function in gram-negative bacteria is reported. Plasmid stability is conferred by the RK2 par locus or by insertion of the spsAB or spsCD symbiotic plasmid stability loci from pNGR234a of Rhizobium sp. NGR234. The vectors carry multiple cloning sites with protection against read-through transcriptional activity of vector sequences. Vector derivatives with the constitutive nptII promoter or a promoterless gusA gene are suitable for the study of gene function or regulation in bacteria.

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Year:  2001        PMID： 11277442     DOI： 10.1094/MPMI.2001.14.3.426

Source DB:  PubMed          Journal:  Mol Plant Microbe Interact        ISSN： 0894-0282            Impact factor:   4.171


  48 in total

1.  Expression of the 1-aminocyclopropane-1-carboxylic acid deaminase gene requires symbiotic nitrogen-fixing regulator gene nifA2 in Mesorhizobium loti MAFF303099.

Authors:  Noriyuki Nukui; Kiwamu Minamisawa; Shin-Ichi Ayabe; Toshio Aoki
Journal:  Appl Environ Microbiol       Date:  2006-07       Impact factor: 4.792

2.  Insights into the extracytoplasmic stress response of Xanthomonas campestris pv. campestris: role and regulation of {sigma}E-dependent activity.

Authors:  Patricia Bordes; Laure Lavatine; Kounthéa Phok; Roland Barriot; Alice Boulanger; Marie-Pierre Castanié-Cornet; Guillaume Déjean; Emmanuelle Lauber; Anke Becker; Matthieu Arlat; Claude Gutierrez
Journal:  J Bacteriol       Date:  2010-10-22       Impact factor: 3.490

3.  The Mesorhizobium loti purB gene is involved in infection thread formation and nodule development in Lotus japonicus.

Authors:  Shin Okazaki; Yoshiyuki Hattori; Kazuhiko Saeki
Journal:  J Bacteriol       Date:  2007-09-07       Impact factor: 3.490

4.  Overexpression of flavodoxin in bacteroids induces changes in antioxidant metabolism leading to delayed senescence and starch accumulation in alfalfa root nodules.

Authors:  Francisco J Redondo; Teodoro Coba de la Peña; César N Morcillo; M Mercedes Lucas; José J Pueyo
Journal:  Plant Physiol       Date:  2008-12-19       Impact factor: 8.340

5.  Function of a chemotaxis-like signal transduction pathway in modulating motility, cell clumping, and cell length in the alphaproteobacterium Azospirillum brasilense.

Authors:  Amber N Bible; Bonnie B Stephens; Davi R Ortega; Zhihong Xie; Gladys Alexandre
Journal:  J Bacteriol       Date:  2008-07-18       Impact factor: 3.490

6.  Roles of predicted glycosyltransferases in the biosynthesis of the Rhizobium etli CE3 O antigen.

Authors:  Kristylea J Ojeda; Laurie Simonds; K Dale Noel
Journal:  J Bacteriol       Date:  2013-02-22       Impact factor: 3.490

7.  In vitro biosynthesis and chemical identification of UDP-N-acetyl-d-quinovosamine (UDP-d-QuiNAc).

Authors:  Tiezheng Li; Laurie Simonds; Evgenii L Kovrigin; K Dale Noel
Journal:  J Biol Chem       Date:  2014-05-09       Impact factor: 5.157

8.  Regulation of a Glycerol-Induced Quinoprotein Alcohol Dehydrogenase by σ54 and a LuxR-Type Regulator in Azospirillum brasilense Sp7.

Authors:  Vijay Shankar Singh; Ashutosh Prakash Dubey; Ankush Gupta; Sudhir Singh; Bhupendra Narain Singh; Anil Kumar Tripathi
Journal:  J Bacteriol       Date:  2017-06-13       Impact factor: 3.490

9.  Identification and regulation of the N-acetylglucosamine utilization pathway of the plant pathogenic bacterium Xanthomonas campestris pv. campestris.

Authors:  Alice Boulanger; Guillaume Déjean; Martine Lautier; Marie Glories; Claudine Zischek; Matthieu Arlat; Emmanuelle Lauber
Journal:  J Bacteriol       Date:  2010-01-15       Impact factor: 3.490

10.  Sinorhizobium meliloti bluB is necessary for production of 5,6-dimethylbenzimidazole, the lower ligand of B12.

Authors:  Gordon R O Campbell; Michiko E Taga; Kavita Mistry; Javier Lloret; Peter J Anderson; John R Roth; Graham C Walker
Journal:  Proc Natl Acad Sci U S A       Date:  2006-03-01       Impact factor: 11.205
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