Literature DB >> 11271826

Detection of feline immunodeficiency provirus by seminested polymerase chain reaction.

V Celer1, H Kulhánková, V Celer1.   

Abstract

Specific primers for the detection of the FIV provirus in peripheral blood mononuclear cells (PBMC) by seminested PCR (snPCR) were developed. Forty cats (patients from veterinary hospitals) were investigated for the presence of FIV serum antibodies by immunoblot and for the presence of provirus in PBMC by snPCR. Seventeen of the 40 examined samples (42.5%) showed the presence of antibodies against FIV. The total number of animals that were found positive in snPCR was 19 (47.5%). Fourteen of the seropositive animals (35%) were positive by snPCR whereas three seropositive animals (7.5%) turned out to be snPCR negative. Of twenty-three animals that were negative by immunoblot, five (12.5%) were found to be positive by snPCR.

Entities:  

Mesh：

Substances：

Year:  2000        PMID： 11271826     DOI： 10.1007/bf02817417

Source DB:  PubMed          Journal:  Folia Microbiol (Praha)        ISSN： 0015-5632            Impact factor:   2.099


  15 in total

1.  Epidemiology of feline leukaemia and feline immunodeficiency virus infections in the Czech Republic.

Authors:  Z Knotek; P Hájková; M Svoboda; M Toman; V Raska
Journal:  Zentralbl Veterinarmed B       Date:  1999-12

2.  Alteration of T-cell subsets in the lymph nodes from cats infected with feline immunodeficiency virus.

Authors:  Y Endo; S Matsumura; T Washizu; T Ishida
Journal:  J Vet Med Sci       Date:  1997-09       Impact factor: 1.267

3.  Delayed seroconversion following naturally acquired caprine arthritis-encephalitis virus infection in goats.

Authors:  E Rimstad; N E East; M Torten; J Higgins; E DeRock; N C Pedersen
Journal:  Am J Vet Res       Date:  1993-11       Impact factor: 1.156

Review 4.  Feline immunodeficiency virus: an interesting model for AIDS studies and an important cat pathogen.

Authors:  M Bendinelli; M Pistello; S Lombardi; A Poli; C Garzelli; D Matteucci; L Ceccherini-Nelli; G Malvaldi; F Tozzini
Journal:  Clin Microbiol Rev       Date:  1995-01       Impact factor: 26.132

5.  gag- and env-specific serum antibodies in cats after natural and experimental infection with feline immunodeficiency virus.

Authors:  G F Rimmelzwaan; K H Siebelink; H Broos; G A Drost; K Weijer; R van Herwijnen; A D Osterhaus
Journal:  Vet Microbiol       Date:  1994-03       Impact factor: 3.293

6.  Retrospective serologic survey for the presence of feline immunodeficiency virus antibody: a comparison of ELISA and IFA techniques.

Authors:  R W Reid; M C Barr; F W Scott
Journal:  Cornell Vet       Date:  1992-10

7.  Simple in vitro methods for titrating feline immunodeficiency virus (FIV) and FIV neutralizing antibodies.

Authors:  F Tozzini; D Matteucci; P Bandecchi; F Baldinotti; A Poli; M Pistello; K H Siebelink; L Ceccherini-Nelli; M Bendinelli
Journal:  J Virol Methods       Date:  1992-06       Impact factor: 2.014

8.  Detection of feline immunodeficiency virus (FIV) nucleic acids in FIV-seronegative cats.

Authors:  S Dandekar; A M Beebe; J Barlough; T Phillips; J Elder; M Torten; N Pedersen
Journal:  J Virol       Date:  1992-07       Impact factor: 5.103

9.  Detection of feline immunodeficiency virus by a nested polymerase chain reaction.

Authors:  E Rimstad; K Ueland
Journal:  J Virol Methods       Date:  1992-03       Impact factor: 2.014

Review 10.  Animal virus receptors.

Authors:  L Jindrák; L Grubhoffer
Journal:  Folia Microbiol (Praha)       Date:  1999       Impact factor: 2.099
View more
  1 in total

1.  Chlamydial infection of cats and human health.

Authors:  M Trávnicek; S Mardzinová; L Cisláková; I Valocký; T Weissová
Journal:  Folia Microbiol (Praha)       Date:  2002       Impact factor: 2.099
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.