Literature DB >> 11271817

Construction of promoter-probe shuttle vectors for Escherichia coli and corynebacteria on the basis of promoterless alpha-amylase gene.

J Ugorcáková1, G Bukovská, J Timko.   

Abstract

We constructed new promoter-probe vectors for E. coli and corynebacteria based on the promoterless alpha-amylase gene originating from Bacillus subtilis. Vectors pJUPAE1 and pJUPAE2 are suitable for isolation of transcriptionally active fragments from plasmids, phages or genomic DNA. alpha-Amylase activity can be easily visually detected on agar plates containing a chromogenic substrate, or by direct measurement of alpha-amylase activity.

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Year:  2000        PMID： 11271817     DOI： 10.1007/bf02817408

Source DB:  PubMed          Journal:  Folia Microbiol (Praha)        ISSN： 0015-5632            Impact factor:   2.099


  13 in total

1.  Construction of new cloning vectors for Brevibacterium lactofermentum.

Authors:  R F Cadenas; C Fernández-González; J F Martín; J A Gil
Journal:  FEMS Microbiol Lett       Date:  1996-03-15       Impact factor: 2.742

2.  A physical and genetic map of the Corynebacterium glutamicum ATCC 13032 chromosome.

Authors:  B Bathe; J Kalinowski; A Pühler
Journal:  Mol Gen Genet       Date:  1996-09-13

3.  Construction and characterization of new corynebacterial plasmids carrying the alpha-amylase gene.

Authors:  J Ugorcáková; M Jucovic; G Bukovská; J Timko
Journal:  Folia Microbiol (Praha)       Date:  1996       Impact factor: 2.099

4.  Multiple sigma factor genes in Brevibacterium lactofermentum: characterization of sigA and sigB.

Authors:  J A Oguiza; A T Marcos; M Malumbres; J F Martín
Journal:  J Bacteriol       Date:  1996-01       Impact factor: 3.490

5.  Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif.

Authors:  M Pátek; B J Eikmanns; J Pátek; H Sahm
Journal:  Microbiology (Reading)       Date:  1996-05       Impact factor: 2.777

6.  Construction and characterization of promoter-probe vectors for Corynebacteria using the kanamycin-resistance reporter gene.

Authors:  R F Cadenas; J F Martín; J A Gil
Journal:  Gene       Date:  1991-02-01       Impact factor: 3.688

7.  Characterization of bacteriophage BFK20 from Brevibacterium flavum.

Authors:  M Koptides; I Barák; M Sisová; E Baloghová; J Ugorcaková; J Timko
Journal:  J Gen Microbiol       Date:  1992-07

8.  Directed mutagenesis of a regulatory palindromic sequence upstream from the Brevibacterium lactofermentum tryptophan operon.

Authors:  C Guerrero; L M Mateos; M Malumbres; J F Martín
Journal:  Gene       Date:  1994-01-28       Impact factor: 3.688

9.  Codon preference in corynebacteria.

Authors:  M Malumbres; J A Gil; J F Martín
Journal:  Gene       Date:  1993-11-30       Impact factor: 3.688

10.  Pulsed-field gel electrophoresis analysis of the genome of amino acid producing corynebacteria: chromosome sizes and diversity of restriction patterns.

Authors:  A Correia; J F Martín; J M Castro
Journal:  Microbiology (Reading)       Date:  1994-10       Impact factor: 2.777
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